缺氧及缺氧复合运动大鼠心
发布时间：2019-07-04 10:05
【摘要】： 平原人进入高原后劳动能力降低,这是对高原低氧环境习服不良在整体水平的表现,积极寻找促进高原习服的措施是当前高原医学与生物学研究的前沿和热点。在平原进行体力锻炼有助于机体对高原缺氧的适应[1],实践表明在高原进行适当的运动训练更有利于促进高原的习服[2]。我们近年的研究表明,在高原低氧环境中进行适当的运动锻炼促进机体对高原低氧环境的习服的机制与缺氧复合运动促进组织毛细血管增生、改善心功能和组织细胞对氧的利用等因素有关[3]。以往在这方面的研究大多是从整体和器官水平上对结构和功能进行观察,而对结构和功能改变的生化和分子生物学基础国内外则报道甚少。高原环境对人的影响的主要因素是缺氧,能量产生和能量利用平衡是进入高原后机体习服与否的关键。因此,我们系统的观察了缺氧复合运动对组织能量代谢的影响,以进一步了解缺氧复合运动促进高原低氧习服的机制。本课题以大鼠在低压舱内游泳训练模拟缺氧复合运动作为动物模型,以能量代谢为中心,运用了生化技术、SDS-PAGE、同位素掺入、western blot技术等多种实验手段,观察了大鼠心肌和骨骼肌肌球蛋白重链异构体组成、葡萄糖有氧和无氧代谢的关键酶、能量监控器AMPK酶活性、线粒体功能等的变化。探讨缺氧习服过程中,适当的运动锻炼促进高原习服,改善机体劳动能力的分子生物学适应机制。为寻求更有效的促习服措施奠定理论基础。主要结果和结论如下: 1.慢性缺氧大鼠血球压积显著增加,右心室肥大;缺氧复合运动后大鼠血球压积与缺氧组比较明显降低,右室指数和右室与左室比值无显著差异;结果表明在高原适当运动可降低红细胞压积,有利于改善微循环状态从而减轻心脏负荷。 2.运动训练大鼠心室肌MHCα异构体比例显著增加;与平原对照和缺氧组比较,缺氧复合运动后MHCα异构体比例显著增加。结果表明运动运动训练可使心肌肌球蛋白异构体组成改变,ATP酶活性高的MHCα异构体比例显著增加,这可能是缺氧条件下适当运动心功能增强的机制之一。 3.慢性缺氧比目鱼肌Ⅰ型MHC显著增加,Ⅱa型MHC显著降低。单纯运动和缺氧复合运动组与缺氧组变化趋势相同。结果表明慢性缺氧和运动训练使比目鱼肌MHC
[Abstract]:After the plain people enter the plateau, the ability to work is reduced, which is the expression of poor acclimatization of the high altitude and low-oxygen environment in the whole level, and the measures to promote the acclimatization of the plateau are the leading edge and the hot spot of the current high-altitude medicine and biology research. Physical exercise in the plain will help the body to adapt to the hypoxia of the plateau[1], and the practice shows that proper exercise training in the plateau is more conducive to the promotion of the acclimatization of the plateau[2]. In recent years, we have shown that proper exercise and exercise in the low-oxygen environment of the plateau promote the mechanism of the acclimatization of the body to the low-oxygen environment of the plateau and the hypoxia-complex exercise to promote the capillary hyperplasia of the tissue, improve the function of the heart function and the utilization of the cell to the oxygen, and so on[3]. In the past, most of the research in this area is the observation of the structure and function from the whole and the organ level, but the biochemical and molecular biological basis of the change of structure and function is very little. The main factor of the influence of the high altitude environment on people is that the balance of oxygen generation, energy generation and energy utilization is the key to the physical training of the body after entering the plateau. Therefore, we have observed the effect of the hypoxia-composite exercise on the energy metabolism of the tissue, so as to further understand the mechanism of the hypoxia-composite exercise to promote the low-oxygen exercise in the plateau. In this paper, the rat's heart and skeletal muscle myosin heavy chain isomers were observed by using a variety of experimental means such as biochemical technology, SDS-PAGE, isotope incorporation, western blot and so on. The key enzymes of the aerobic and anaerobic metabolism of glucose, the activity of AMPK in the energy monitor, the function of the mitochondria, etc. In order to improve the mechanism of molecular biology, the proper exercise of exercise can promote the acclimatization of the plateau and improve the working ability of the body in the course of the hypoxic acclimatization. To lay a theoretical foundation for the search for more effective training measures. The main results and conclusions are as follows: 1. There was a significant increase in the hematocrit and right ventricular hypertrophy in the rats with chronic hypoxia. There is no significant difference in left ventricular ratio. The results show that the proper movement of the plateau can lower the hematocrit, which is beneficial to the improvement of microcirculation. in ord to reduce that load of the heart.2. the proportion of the MHC class of the ventricular muscle in the exercise-trained rat was significantly increased; compared with the plain control and the oxygen-deficient group, the ratio of the MHC-isomer of the ventricular muscle of the rat The results show that the activity of the MHCs can be changed by the exercise training, and the ratio of the MHC to the high activity of the ATPase is obviously increased, which may be One of the mechanisms for proper exercise of cardiac function under the condition of hypoxia. The type of MHC class 鈪，
本文编号：2509840
[Abstract]:After the plain people enter the plateau, the ability to work is reduced, which is the expression of poor acclimatization of the high altitude and low-oxygen environment in the whole level, and the measures to promote the acclimatization of the plateau are the leading edge and the hot spot of the current high-altitude medicine and biology research. Physical exercise in the plain will help the body to adapt to the hypoxia of the plateau[1], and the practice shows that proper exercise training in the plateau is more conducive to the promotion of the acclimatization of the plateau[2]. In recent years, we have shown that proper exercise and exercise in the low-oxygen environment of the plateau promote the mechanism of the acclimatization of the body to the low-oxygen environment of the plateau and the hypoxia-complex exercise to promote the capillary hyperplasia of the tissue, improve the function of the heart function and the utilization of the cell to the oxygen, and so on[3]. In the past, most of the research in this area is the observation of the structure and function from the whole and the organ level, but the biochemical and molecular biological basis of the change of structure and function is very little. The main factor of the influence of the high altitude environment on people is that the balance of oxygen generation, energy generation and energy utilization is the key to the physical training of the body after entering the plateau. Therefore, we have observed the effect of the hypoxia-composite exercise on the energy metabolism of the tissue, so as to further understand the mechanism of the hypoxia-composite exercise to promote the low-oxygen exercise in the plateau. In this paper, the rat's heart and skeletal muscle myosin heavy chain isomers were observed by using a variety of experimental means such as biochemical technology, SDS-PAGE, isotope incorporation, western blot and so on. The key enzymes of the aerobic and anaerobic metabolism of glucose, the activity of AMPK in the energy monitor, the function of the mitochondria, etc. In order to improve the mechanism of molecular biology, the proper exercise of exercise can promote the acclimatization of the plateau and improve the working ability of the body in the course of the hypoxic acclimatization. To lay a theoretical foundation for the search for more effective training measures. The main results and conclusions are as follows: 1. There was a significant increase in the hematocrit and right ventricular hypertrophy in the rats with chronic hypoxia. There is no significant difference in left ventricular ratio. The results show that the proper movement of the plateau can lower the hematocrit, which is beneficial to the improvement of microcirculation. in ord to reduce that load of the heart.2. the proportion of the MHC class of the ventricular muscle in the exercise-trained rat was significantly increased; compared with the plain control and the oxygen-deficient group, the ratio of the MHC-isomer of the ventricular muscle of the rat The results show that the activity of the MHCs can be changed by the exercise training, and the ratio of the MHC to the high activity of the ATPase is obviously increased, which may be One of the mechanisms for proper exercise of cardiac function under the condition of hypoxia. The type of MHC class 鈪，
本文编号：2509840
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