Impaired balance of T helper 17/T regulatory cells in carbon

发布时间：2022-10-09 20:16

　　AIM:To investigate the effect of T helper（Th）17/T regulatory（Treg）cells on hepatic fibrosis in mice and its possible mechanism.METHODS:Hepatic fibrosis was induced by intraperitoneal injection of carbon tetrachloride.Hepatic pathological changes were observed by hematoxylin and eosin staining;the protein levels of interleukin（IL）-6,transforming growth factor（TGF）-βandα-smooth muscle actin（SMA）in liver tissue were determined by Western blotting;and the frequency of Th17 and Treg cells in the live... 

【文章页数】：9 页

【文章目录】：
INTRODUCTION
MATERIALS AND METHODS
    Animals
    Liver fibrosis model and sample collection
    Histological examination
    Western blotting
    Flow cytometric analysis of T cell subsets
    Isolation of HSCs
    Preparation of Th17 and Treg cells
    Cell co-culture
    Immunofluorescence staining
    Statistical analysis
RESULTS
    Histopathology of liver tissue
    Expression of IL-6, TGF-βandα-SMA in liver tissue
    Increased Th17 cells and decreased Treg cells in CCl4-induced hepatic fibrosis model
    Th17/Treg ratios indicated severity of fibrosis in mice
    Effect of Th17/Treg cells on activation of HSCs
DISCUSSION



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