华支睾吸虫感染后血清IL-33检测及其意义

发布时间：2018-07-03 17:07

本文选题：华支睾吸虫 + 白细胞介素-　；参考：《齐齐哈尔医学院学报》2016年31期

【摘要】：目的探讨华支睾吸虫感染后血清白细胞介素-33(IL-33)的检测及临床意义。方法建立不同品系小鼠华支睾吸虫感染模型,采用ELISA法对感染组和正常对照组小鼠血清中IL-33水平进行测定。同时检测45例华支睾吸虫感染患者及16名健康对照者血清中IL-33水平。结果感染华支睾吸虫后,小鼠血清中IL-33水平明显增高,且随感染时间的增加而逐渐增高。感染14 d时,病理损伤严重的FVB小鼠血清中IL-33水平明显高于病理损伤较轻的Balb/c小鼠,差异有统计学意义(P0.05)。感染28d时,两种品系小鼠血清中IL-33水平显著高于感染0d时,差异有统计学意义(P0.01)。此外,与健康对照相比,华支睾吸虫感染患者血清中IL-33水平也明显增加(P0.01)。结论华支睾吸虫感染后,血清中IL-33水平升高,可作为辅助诊断的参考指标。
[Abstract]:Objective to investigate the detection and clinical significance of serum interleukin-33 (IL-33) after Clonorchis sinensis infection. Methods the infection models of Clonorchis sinensis were established in different strains of mice. The serum IL-33 levels of infected mice and normal control mice were determined by Elisa. The serum levels of IL-33 in 45 patients with Clonorchis sinensis infection and 16 healthy controls were also detected. Results after infection with Clonorchis sinensis, the level of IL-33 in serum of mice increased significantly, and gradually increased with the increase of infection time. After 14 days of infection, the level of IL-33 in serum of FVB mice with severe pathological injury was significantly higher than that of Balb / r / c mice with mild pathological injury (P0.05). The levels of IL-33 in serum of the two strains were significantly higher than those on the day 0 after infection (P0.01). In addition, the serum level of IL-33 in patients with clonorchiasis sinensis was significantly higher than that in healthy controls (P0.01). Conclusion the level of IL-33 in serum increased after Clonorchis sinensis infection and can be used as a reference index for auxiliary diagnosis.
【作者单位】：徐州医科大学病原生物学与免疫学教研室;徐州医科大学感染与免疫实验室;
【基金】：国家自然科学基金青年科学基金项目(31302077) 2015年度江苏省脑病生物信息重点实验室开放研究课题(1506) 2016年度江苏省高校自然科学面上项目(16KJB310016)
【分类号】：R532.23

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