2940nm Er：YAG联合儿茶素治疗鼠皮肤光老化的实验研究

发布时间：2018-01-01 01:03

本文关键词：2940nm Er：YAG联合儿茶素治疗鼠皮肤光老化的实验研究　出处：《南昌大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：探究2940nm Er：YAG激光、儿茶素抗鼠皮肤光老化作用，进一步探讨两者对于光老化可能的作用机理。方法：根据不同的处理方法，将60只昆明小鼠为5组：A（对照组）、B（光老化组）、C（光老化+激光组）、D （光老化+儿茶素组）、E（光老化+激光+儿茶素组），每组各12只，对干预（除对照组）小鼠行持续8周紫外线照射、持续4周儿茶素、激光处理后，应用HE、免疫组化、Western blot及生物检测等方法对各组小鼠皮肤结构、超氧化物岐化酶SOD活力、丙二醛MDA含量、Ⅰ型胶原平均光密度AOD及MMP-1蛋白表达量进行检测。结果： B（光老化组）小鼠皮肤肉眼观干燥、粗糙、皮沟明显、色素沉着明显；HE染色显示小鼠皮肤表皮层增厚厚度不均一，角质层增生偶可见脱落；真皮层胶原减少，厚度变薄，纤维层断裂，胶原纤维变粗条索化，腺体增生；与其余各组小鼠相比SOD活力明显降低（P㩳0.01），MDA含量明显增多（P㩳0.01）,Ⅰ型胶原免疫组化见真皮层新生胶原减少，真皮层显著变薄，平均光密度AOD明显降低（P㩳0.01）；MMP-1蛋白表达明显增高（P㩳0.01）。与光老化组相比，各干预组小鼠皮肤均有明显改善，皮肤较光滑、红润、光泽度好、细腻、动态性皱纹基本消失、无明显色素沉着；HE染色可见表皮层结构完整，但仍可见角质层脱落，真皮层变厚，可见新生胶原，腺体稍增多，排列较均匀；SOD活力明显升高（＊P㩳0.01；◆P㩳0.05）；MDA含量明显下降，均具有显著性差异（＊P㩳0.01）,Ⅰ型胶原免疫组化见真皮层新生胶原增多，真皮层显著增厚，平均光密度AOD明显升高（＊P㩳0.01）；MMP-1蛋白表达明显减少（＊P㩳0.01）。 E与A、D组相较MMP-1表达量具有统计学意义（◆P㩳0.05；＊P㩳0.01），E与C组相较SOD活力值、MDA含量均有显著性差异（＊P㩳0.01）。结论： 2940nm Er：YAG激光、儿茶素均可促进皮肤紧致年轻化、刺激胶原再生、增强SOD活性、降低MDA含量，减少MMP-1蛋白表达，两者联合相较于单独使用激光、儿茶素疗效更佳，，可能为效果的叠加，这值得进一步深入研究，为临床治疗和研究提供可靠依据。
[Abstract]:Objective: The effects of catechin on photoaging of mouse skin were investigated by 2940nm Er:YAG laser, and the possible mechanism of photoaging was discussed. Methods: According to different treatment methods, 60 Kunming mice were divided into 5 groups (control group): control group (control group) (photoaging group) (photoaging laser group) (photoaging group). E- (photoaging laser catechin group, 12 rats in each group) were treated by ultraviolet irradiation for 8 weeks and catechin for 4 weeks. After laser treatment, HEH and immunohistochemistry were used. The skin structure, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of mice were determined by Western blot and bioassay. The average optical density of type I collagen AOD and MMP-1 protein expression were detected. Results: B (light aging group) mice skin dry, rough, skin sulcus obvious, pigmentation; He staining showed that the thickness of the skin epidermis was not uniform, and the exfoliation of the cuticle was occasionally seen. The cortical collagen decreased, the thickness became thinner, the fibrous layer was broken, the collagen fibers became thicker and striped, and the glands proliferated. Compared with the other groups, the activity of SOD decreased significantly. The content of MDA increased significantly (P < 0.05). The immunohistochemical staining of type I collagen showed that the new collagen in the dermis decreased, the cortex thinned significantly, and the mean optical density (AOD) decreased significantly. The expression of MMP-1 protein increased significantly. 0.01g. Compared with the light aging group, the skin of each intervention group was obviously improved, the skin was smooth, ruddy, glossy, delicate, dynamic wrinkles disappeared basically, no obvious pigmentation. The structure of the epidermis was intact in HE staining, but the cuticular layer was shedding, the dermis became thicker, the new collagen was visible, the glands increased slightly, and the arrangement was even. The activity of SOD increased significantly. 0.01; P? The content of MDA decreased obviously, and there was significant difference in the content of MDA between 0. 05% and 0. 05% (P < 0. 05). The immunohistochemical staining of type I collagen showed the increase of new collagen in the dermis, the thickening of the dermis, and the increase of the average optical density (AOD). The expression of MMP-1 protein decreased significantly. 0.01g. The expression of MMP-1 in E group was significantly higher than that in Agna D group (P? 0.05? There was significant difference in SOD activity between group E and group C (P < 0.01). 0.01g. Conclusion: At 2940nm Er:YAG laser, catechins could promote skin aging, stimulate collagen regeneration, enhance SOD activity, decrease MDA content and decrease MMP-1 protein expression. Compared with laser alone, catechin has better curative effect and may be the superposition of effect, which is worthy of further study and provides reliable basis for clinical treatment and research.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R62

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