孕酮对新生鼠脑室周围白质软化模型脑白质少突细胞标记物O1和O4表达的影响

发布时间：2018-01-27 13:34

本文关键词： 孕酮脑室周围白质软化少突细胞标记物O 少突细胞标记物O 新生大鼠　出处：《临床儿科杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨孕酮对新生鼠脑室周围白质软化(PVL)动物模型脑室周围白质O1和O4表达的影响。方法 2日龄新生SD大鼠随机分为模型组、实验组、假手术组。模型组和实验组结扎左侧颈总动脉并吸入低氧气源(8%O2+92%N2)0.5 h制备PVL动物模型,实验组脑缺氧缺血后立即腹腔注射孕酮10 mg/(kg·d);假手术组只分离左侧颈总动脉不结扎、不缺氧。术后1、4、7、14天比较三组脑组织病理改变,免疫组织化学染色方法检测三组大鼠各个时点脑室旁白质区O1、O4表达。结果假手术组各时点脑白质区未见异常病理改变;模型组和实验组均出现左侧脑室扩大和脑室周围白质软化灶等病理改变,但实验组各时点脑白质病理改变均明显轻于模型组。三组大鼠脑室旁白质区O1、O4阳性细胞的积分光密度(IOD)在术后1、4、7天的表达逐渐增加,第7天达高峰,第14天下降,差异有统计学意义(P均0.01)。在术后1、4、7、14天,三组脑室旁白质区O 1、O 4阳性细胞IOD在同一时间点均以假手术组最多,实验组其次,模型组最少,差异有统计学意义(P0.01)。结论孕酮可减轻新生大鼠PVL模型动物脑室旁白质区病理损伤,促进脑室周围白质O1、O4表达,促进少突胶质细胞分化成熟。
[Abstract]:Objective to investigate the progesterone on neonatal rats with periventricular leukomalacia (PVL) effect of the animal model of ventricle white matter around the O1 and O4 expression. Methods 2 day old SD rats were randomly divided into model group, experimental group, sham operation group, model group and experimental group left common carotid artery ligation and inhalation of low oxygen source (8%O2+92%N2) the animal model of PVL 0.5 h preparation, the experimental group immediately after cerebral ischemia by intraperitoneal injection of progesterone 10 mg/ (kg - D); sham operation group only separated the left common carotid artery ligation and hypoxia. The change of brain tissue 1,4,7,14 days compared three groups of postoperative pathology, immunohistochemical staining method using three groups of rats each time the periventricular region of O1, the expression of O4. Results in the sham operation group were cerebral white matter abnormal pathological changes; model group and experimental group were changed left ventricular enlargement and periventricular leukomalacia lesions were pathological, but the experimental group of brain white matter pathology Changes were significantly lighter than the model group. Three groups of rats with periventricular area O1, the integral optical density of O4 positive cells (IOD) increased expression in 1,4,7 days after operation, seventh Tianda peak, fourteenth days decreased, the difference was statistically significant (P < 0.01). After 1,4,7,14 days, the three groups brain white matter area room O 1 O 4 IOD positive cells at the same time points were in sham operation group, model group, experimental group second, at least, the difference was statistically significant (P0.01). Conclusion progesterone can reduce the PVL animal model of periventricular area pathological injury in neonatal rats, promote the periventricular white matter O1 the expression of O4, promote oligodendrocyte differentiation and maturation.

【作者单位】：遵义医学院附属医院新生儿科;
【基金】：贵州省卫计委资助课题(No.gzwjkj2014-1-072) 遵义医学院硕士点基金资助课题(No.2C2201603186)
【分类号】：R722.1
【正文快照】： 随着新生儿重症监护病房(NICU)救治水平的提升,极低出生体质量儿、超低出生体质量儿的存活率提高,早产儿脑损伤发病率也逐渐增加。脑室周围白质软化(periventricular leukomalacia,PVL)是早产儿脑损伤的主要形式,是导致早产儿神经发育、行为障碍及脑瘫的主要原因[1]。PVL发病

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