巨细胞病毒感染后新生小鼠脑内Wif-1、Shh的表达变化

发布时间：2018-01-31 06:24

本文关键词： 巨细胞病毒神经异常Wif-蛋白 Shh蛋白细胞周期小鼠　出处：《神经解剖学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:探讨鼠巨细胞病毒(murine cytomegalovirus,MCMV)感染后新生小鼠脑内Wnt inhibitory factor-1(Wif-1)、Sonic hedgehog(Shh)信号分子的表达变化及对中枢神经系统发育的可能影响。方法:体外3T3细胞培养、MCMV接种增殖;60只生后3 d的BALB/c仔鼠随机分为正常对照组及病毒感染组,病毒组通过腹腔注射法建立新生小鼠中枢神经系统MCMV感染模型,而对照组则注射等量的生理盐水;每组设立3个时间点亚组(造模后3 d,7 d,14 d),相应时点处死仔鼠,取脑组织备用。通过RT-PCR检测两组各时点脑组织中的MCMV-DNA表达;Western Blot检测小鼠脑组织中Wif-1、Shh蛋白的表达;流式细胞术检测两组的细胞周期比例改变。结果:病毒组小鼠脑组织中MCMV-DNA的表达随着时间推移持续增多,而对照组3个时间点均阴性表达;病毒组小鼠脑组织中Wif-1分子的表达相对于对照组增强,而Shh分子的表达相比于对照组则表现出明显的抑制;病毒组小鼠脑组织中处于分裂期的细胞百分比较对照组下降。结论:MCMV感染后的新生小鼠脑组织内Wif-1表达增强,而Shh信号分子则下调,其表达异常变化可能影响Wnt、Hedgehog信号通路,并影响中枢神经系统成熟过程。
[Abstract]:Objective: to study murine cytomegalovirus of murine cytomegalovirus. Wnt inhibitory factor-1 and Wif-1 were found in the brain of newborn mice after MCMV infection. Changes of signal molecule expression in Sonic hedgehog and its possible effect on the development of central nervous system methods: 3T3 cells cultured in vitro were inoculated and proliferated. Sixty postnatal BALB/c mice were randomly divided into normal control group and viral infection group. The MCMV infection model of central nervous system of newborn mice was established by intraperitoneal injection in the virus group. The control group was injected with the same amount of normal saline. Each group was divided into 3 subgroups (3 days, 7 days and 14 days after modeling), and the rats were killed at the corresponding time points. RT-PCR was used to detect the expression of MCMV-DNA in brain tissue at different time points. Western Blot was used to detect the expression of Wif-1 protein in mouse brain. Results: the expression of MCMV-DNA in the brain tissue of mice in the virus group increased with the passage of time, but the expression of MCMV-DNA in the control group was negative at three time points. Compared with the control group, the expression of Wif-1 in the brain tissue of the virus group was increased, while the expression of the Shh molecule was significantly inhibited compared with the control group. Compared with the control group, the percentage of cells in the brain tissue of the virus group decreased. Conclusion the expression of Wif-1 in the brain tissue of newborn mice infected with MCMV is increased, while the Shh signal molecule is down-regulated. The abnormal changes of its expression may affect the Hedgehog signaling pathway and the maturation process of the central nervous system (CNS).
【作者单位】：徐州医科大学附属医院儿科;
【基金】：江苏省六大人才高峰资助项目(2011-WS-075)
【分类号】：R722.1
【正文快照】： 巨细胞病毒(cytomegalovirus,CMV)感染在人群中极为普遍,但因为其毒力相对较低,对于免疫功能完善的成年人大多数时候表现为无症状性感染[1]。然而,在免疫功能缺陷及不完善的人中,常会有非常严重的并发症,尤其是胎儿期的宫内感染及婴儿期的感染[2]。CMV感染可累及患儿多个系统,

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