精神分裂症患者听觉惊跳反射抑制与儿茶酚-O-甲基转移酶基因Val158Met多态性的相关性

发布时间：2018-01-31 09:51

本文关键词： 精神分裂症惊跳反射抑制儿茶酚-O-甲基转移酶多态性　出处：《中国心理卫生杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨精神分裂症患者儿茶酚-O-甲基转移酶(COMT)第158位密码子从缬氨酸到蛋氨酸的多态性(Vall58Met)与听觉惊跳反射抑制(PPI)的关系。方法:选取符合美国精神障碍诊断与统计手册第4版(DSM-IV)的精神分裂症患者178例,正常对照190例,使用SR-HLAB惊跳反射监控系统测查听觉惊跳反射,其分析指标包括:惊跳反射的反应波幅(SR);惊跳反射的适应性(HAB);时间间隔(LI)为30 ms、60 ms、120 ms时的听觉刺激惊跳反射弱刺激抑制(PPI30%、PPI60%、PPI120%);应用聚合酶链反应和限制性片段长度多态性的方法,分析精神分裂症组与对照组COMT Vall58Met基因型与等位基因分布频率。结果:精神分裂症组的波幅(SR)低于对照组[(563±460)mVvs.(695±447)m V,P0.05],适应性(HAB)低于对照组[(32±46)vs.(48±33),P0.01],差异有统计学意义;精神分裂症组与对照组之间PPI差异有统计学意义(F=7.15,P0.05),组与时间间隔的交互作用差异有统计学意义(F=5.57,P0.05),进一步分析发现精神分裂症组的%PPI120低于对照组[(27±5)vs.(35±3),P0.05]。2组间COMT基因型和等位基因分布有统计学意义(χ~2=8.16、11.74,均P0.05)。COMT三种基因型对HAB%的主效应有统计学意义(F=3.07,P0.05);分组和COMT基因型对SR,HAB%,%PPI120的交互作用无统计学意义(F=1.64、2.87、2.26,均P0.05)。结论:COMT基因Vall58Met多态性可能与精神分裂症的适应性有关,但与精神分裂症PPI缺陷可能无关。
[Abstract]:Objective: to investigate the polymorphism of codon 158 from valine to methionine (Vall58 Metals) and auditory agitation reflex inhibition (ASRR) in schizophrenic patients with catechol-O-methyltransferase (COMT). Methods: 178 schizophrenic patients who were in accordance with the fourth edition of the American Handbook for the diagnosis and Statistics of Mental Disorders (DSM-IV) were selected. In the normal control group, SR-HLAB reflex monitoring system was used to detect the auditory shock reflex. The analysis indexes included: the response amplitude of the shock reflex; The adaptability of jumping reflex to HABN; The auditory stimuli at intervals of 30 Ms ~ 60 Ms ~ 120 Ms inhibited PPI30 and PPI _ (60) and PPI _ (120). Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used. The frequency of COMT Vall58Met genotype and allele distribution in schizophrenia group and control group were analyzed. Results: the amplitude of COMT Vall58Met in schizophrenia group was lower than that in control group. [563 卤460mVvs.695 卤447mVV (P0.05), and the adaptive HABs were lower than those in the control group (P < 0.05). [The difference was statistically significant (32 卤46 vs. 48 卤33 P0.01). The difference of PPI between the schizophrenia group and the control group was statistically significant (P 0.05), and the interaction between the group and the time interval was significantly different (P = 5.57). Further analysis showed that PPI120 in schizophrenia group was lower than that in control group. [The distribution of COMT genotypes and alleles was statistically significant (蠂 ~ 2 = 8.16, 11.74) among the groups (27 卤5) vs.35 卤3 (P0.05). The main effect of all three genotypes on HB% was significant (P < 0.05). There was no significant difference in the interaction between the subgroup and COMT genotype on PPI120. Conclusion the Vall58Met polymorphism of the 1: COMT gene may be related to the adaptability of schizophrenia, but it may not be related to the PPI deficiency in schizophrenia.
【作者单位】：北京回龙观医院;北京大学第六医院北京大学精神卫生研究所卫生部精神卫生学重点实验室(北京大学);
【分类号】：R749.3
【正文快照】： 京大学),北京100191通信作者:谭云龙yltan21@126.com;周东丰Zhoudf@bjmuedu.cn)(中国心理卫生杂志,2017,31(6):436-441.)Corresponding author:TAN Yun-Long,yltan21@126.com;ZHOU Dong-Feng,Zhoudf@bjmuedu.cnCOMT Vall58M et polymorphism w as genotyped by polymerase cha

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