UUO幼年大鼠肾组织基质细胞衍生因子-1蛋白表达趋势及意义

发布时间：2018-02-19 23:14

本文关键词： 基质细胞衍生因子-1 转化生长因子-β1 肾小管间质纤维化单侧输尿管梗阻　出处：《山西医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：探讨幼鼠肾间质纤维化模型(单侧输尿管结扎模型)中基质细胞衍生因子-1(SDF-1)不同时间点的表达趋势、相关性,以及用阿托伐他汀干预的效应。方法：建立幼年大鼠肾间质纤维化模型。将90只幼鼠随机分为对照组、药物组和模型组,每组各30只。各组分别于实验第1d、3d、5d、7d、14d各时间点取肾组织进行相关指标测定。采用Masson的染色方法评价各组个时间点肾小管受损的程度。免疫组化法检测大鼠肾组织中SDF-1和TGF-β1的蛋白表达趋势及相关性。用SPSS13.0统计软件进行统计学处理。结果：镜下观察UUO幼鼠肾间质纤维化过程中SDF-1表达于肾小管上皮细胞,SDF-1在正常幼鼠肾组织中表达低微,而在肾纤维化过程中其表达水平明显升高,而且随着幼鼠肾纤维化程度的加重相应地表达逐渐增高。对照组,模型组和用药组的SDF-1灰度值相比较,显示三组之间存在差别(F=9.04,P=0.001),用药组灰度值高于模型组,其纤维化程度低于模型组；三组各个时间点的比较结果显示,模型组(F=70.86,P0.001)和用药组(F=92.28,P0.001)不同时间点的灰度值之间存在差别,模型组与用药组在各时间点间的灰度值比较结果除了第一天差别无统计学意义之外(P0.05),其余各时间点之间均存在差别(P0.05)。TGF-β1的结果与SDF-1类似。实验显示随着肾纤维化程度的越重,TGF-β1表达趋势越高,SDF-1蛋白表达趋势越多,二者之间可能存在着相互作用。SDF-1蛋白表达趋势与肾纤维化病变程度呈正相关,而与各时间点各组的灰度值呈负相关,即SDF-1蛋白表达趋势越高,纤维化程度越重,其灰度值越低。结论：SDF-1与肾间质纤维化有着密切的关系,SDF-1的表达上调,可能通过干预促纤维化因子TGF-β1的变化抑制了肾间质纤维化的进展。阿托伐他汀保护肾脏可能是通过下调SDF-1的表达实现的。
[Abstract]:Objective: to investigate the expression trend and correlation of stromal cell derived factor-1 SDF-1 in renal interstitial fibrosis model of young rats (unilateral ureteral ligation model) and the effect of Atto vastatin intervention. Methods: the renal interstitial fibrosis model of juvenile rats was established. 90 young rats were randomly divided into control group, drug group and model group. There were 30 rats in each group. The renal tissues were taken from each group on the 1st day, 3d, 5d, 7d and 14d, respectively. The degree of renal tubule damage was evaluated by Masson staining method. The renal tissue of rats was detected by immunohistochemical method. The expression trend and correlation of SDF-1 and TGF- 尾 1 were analyzed by SPSS13.0 software. Results: the expression of SDF-1 in renal tubuloepithelial cells of UUO young rats was observed to be low in normal renal tissues, but significantly increased in the process of renal fibrosis in the process of renal interstitial fibrosis in young UUO rats, and the expression of SDF-1 in renal tubuloepithelial cells was significantly increased in the process of renal fibrosis. The SDF-1 grayscale values of control group, model group and medication group were compared with each other, which showed that there was a difference between the three groups, and the gray value of drug group was higher than that of model group, and that of the control group was higher than that of the control group, and that of the model group was higher than that of the control group, and that of the model group was higher than that of the control group. The degree of fibrosis in the model group was lower than that in the model group, and the results of comparison between the three groups at different time points showed that there were differences in gray values between the model group and the medication group at different time points. The results of gray value comparison between the model group and the medication group at each time point were similar to that of SDF-1, except that there was no significant difference on the first day (P 0.05). The results of the other time points were similar to that of SDF-1. The higher the degree of TGF- 尾 1 expression, the higher the expression trend of SDF-1 protein. There may be a positive correlation between the expression trend of SDF-1 protein and the degree of renal fibrosis, but a negative correlation with the gray value of each group at each time point, that is, the higher the expression trend of SDF-1 protein is, the more serious the fibrosis degree is, and the lower the gray value is. Conclusion there is a close relationship between the expression of SDF-1 and renal interstitial fibrosis. The change of TGF- 尾 1 may inhibit the progress of renal interstitial fibrosis. Atto vastatin may protect the kidney by down-regulating the expression of SDF-1.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R726.9

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