小学生携带甲氧西林耐药金葡菌及其毒素基因分析

发布时间：2018-02-23 13:40

  本文关键词： 甲氧西林抗药性 葡萄球菌 金黄色 基因 学生　出处：《中国学校卫生》2017年04期 　论文类型：期刊论文

【摘要】：目的了解小学生携带甲氧西林耐药金葡菌(MRSA)情况及菌株中毒素基因,为靶向性药物研发等医学实践提供遗传标志物资料。方法方便整群抽取广州市1所小学在校健康小学生1 012名,进行鼻拭子采样,采用PCR实验检测菌株中毒素基因。结果小学生中金葡菌携带率为40.1%,MRSA携带率为1.2%。金葡菌中hlb,sak,scn,chp,sep基因的检出率较高(81.7%,97.8%,82.4%,77.0%),其次是seA,seB,seC基因(33.4%,15.1%, 7.2% )。MRSA菌株中的chp,seB,seC,pvl基因的检出率均明显高于甲氧西林敏感金葡菌(MSSA)菌株(P值均0.05);MRSA菌株中同时检出2种或3种毒素基因的检出率均明显高于MSSA菌株(P值均0.05)。结论健康小学生金葡菌及其毒素基因携带率较高,MRSA菌株的潜在致病能力不容忽视。
[Abstract]:Objective to investigate the prevalence of MRSA-carrying methicillin resistant Staphylococcus aureus in primary school students and the toxin gene in the strain. Methods 1012 healthy pupils in a primary school in Guangzhou were sampled by cluster sampling for nasal swabs. The PCR test was used to detect the toxin genes of the strains. Results the carrying rate of Staphylococcus aureus in primary school students was 40.1 and the rate of carrying MRSA was 1.2. The detection rate of hlbbsaksakscnscnscnscnchpfsep in Staphylococcus aureus was higher than that of 81.77.87.87.87.87.87.87.87.80.The next was the gene 33.41.The detection rate of chpseBseBseCpvl in 7.2%% MRSA was all clear. The results showed that the detection rate of the toxin gene of Staphylococcus aureus in primary school students was 40.1%. The detection rate of chpseBseBseCpvl gene in 7.2% strains of Staphylococcus aureus was clear. The detection rate of 2 or 3 toxin genes in MRSA strains was significantly higher than that in MSSA strains. Conclusion the positive rate of staphylococcus aureus and its toxin gene in healthy primary school students is higher than that of MSSA strains. The potential pathogenicity of higher MRSA strains should not be ignored.
【作者单位】： 广东药科大学公共卫生学院流统系;
【基金】：国家自然科学基金项目(81602901) 广东大学生科技创新培育专项(pdjh2016a0256) 2015年广东药学院大学生创新创业培育项目
【分类号】：R725.1;R446.5
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