气相色谱-三重四级杆质谱法测定肥胖儿童血清中6种邻苯二甲酸酯

发布时间：2018-02-23 21:31

本文关键词： 气相色谱-三重四级杆质谱法肥胖儿童血清邻苯二甲酸酯　出处：《现代预防医学》2017年18期 　论文类型：期刊论文

【摘要】：目的建立气相色谱-三重四级杆质谱法(GC-QqQ MS)测定肥胖儿童血清中6种邻苯二甲酸酯类(PAEs)。方法血清样品经乙醇沉定蛋白、正己烷提取后,利用GC-QqQ MS进行测定。结果 6种PAEs(邻苯二甲酸二甲酯,邻苯二甲酸二乙酯,邻苯二甲酸二丁酯,邻苯二甲酸丁基苄基酯,邻苯二甲酸(2-乙基己基)酯,邻苯二甲酸二正辛酯)在1~200μg/L浓度范围内线性良好,相关系数(r值)均大于0.999。终浓度分别为5、25和50μg/L的基质加标实验显示6种PAEs平均回收率在79.1%~93.0%之间,相对标准偏差(RSD%,n=3)在1.4%~6.9%之间。方法定量限低于现有的相关报道,其中DBP和DEHP的最低定量限分别为0.5μg/L和0.8μg/L,其余PAEs最低定量限为0.2μg/L。肥胖儿童血清测定结果显示,血清中不同程度检出PAEs,其中DEHP的检出率和含量范围分别为100%和10μg/L~400μg/L(n=30)。结论该法前处理选择性好、灵敏度高、简单、快速,适用于肥胖儿童血清中PAEs的测定。
[Abstract]:Objective to establish a gas chromatography-quadruplex mass spectrometry (GC-QQQ MS) method for the determination of six phthalates in the serum of obese children. Methods the serum samples were extracted with ethanol precipitated protein and n-hexane. Results six kinds of PAEs (dimethyl phthalate, diethyl phthalate, dibutyl phthalate, Ding Ji benzyl phthalate, 2-ethylhexyl phthalate) ester were determined by GC-QqQ MS. The linear range of dioctyl phthalate was 1 渭 g / L, the correlation coefficient (r) was greater than 0.999.The results showed that the average recoveries of 6 PAEs were between 79.1% and 93.0% when the final concentrations were 5 渭 g / L and 50 渭 g / L, respectively. The quantitative limit of DBP and DEHP were 0.5 渭 g / L and 0.8 渭 g / L respectively, and the lowest limit of PAEs was 0.2 渭 g / L in obese children. The detection rate and content range of DEHP in serum were 100% and 10 渭 g / L respectively. Conclusion this method has good selectivity, high sensitivity, simplicity and rapidity, and is suitable for the determination of PAEs in serum of obese children.
【作者单位】：常州市疾病预防控制中心食源性与环境疾病监测防治所;南京医科大学公共卫生学院卫生检验学系;
【基金】：江苏省高校优势学科建设工程资助项目(PAPD2010)
【分类号】：R723.14

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