脑源性神经营养因子与儿童哮喘的相关性研究

发布时间：2018-02-26 20:24

本文关键词： 血清脑源性神经营养因子哮喘严重程度儿童　出处：《石河子大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察血清脑源性神经营养因子(BDNF)在儿童哮喘中的表达水平,探讨BDNF在儿童哮喘发病机制中的可能作用及其与哮喘患儿病情严重程度的关系。方法:哮喘组选取2015年7月至2016年7月于石河子大学医学院第一附属医院儿科诊治的60例哮喘急性期患儿,其中男性39例,女性21例,年龄1.3~14.0岁,平均(8.1±3.2)岁;将哮喘患儿按哮喘病情严重程度分为轻度组18例、中度组25例和重度组17例;对照组选取同期本院体检的健康儿童60例,其中男性38例、女性22例,年龄1.1~14.4岁,平均(7.4±3.2)岁,并收集临床基本资料。哮喘组儿童分别于发作期治疗前和症状缓解期(无喘息症状、肺部未闻及哮鸣音、予以规范化控制治疗)抽取空腹静脉血各2ml,对照组于就诊当天取空腹外周静脉血2ml,室温(20~25℃)60min后3000r/min离心10min,取血清分装于EP管中,-70℃冰箱留存待检,避免反复冻融。应用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测两组儿童血清中BDNF的表达水平,比较不同严重程度的哮喘患儿血清BDNF水平的差异,分析BDNF水平与儿童哮喘病情严重程度的关系,同时将收集的临床基本资料进行统计学分析。数据采用SPSS 17.0统计软件进行统计学处理。计量资料以均数±标准差(?x±s)表示,正态分布且方差齐者采用t检验或方差分析,方差不齐者采用非参数检验;计数资料以率表示,采用卡方检验进行比较;变量间的相关性采用相关性分析。P0.05为差异有统计学意义。结果:1.哮喘急性发作组及症状缓解组的BDNF水平分别为(8.8±0.6)ng/ml、(6.4±0.9)ng/ml,均明显高于对照组(1.9±1.2)ng/ml,以急性发作组最高(P0.05),缓解期的BDNF水平低于发作期(P0.05)。2.哮喘患儿病情严重程度不同,其血清BDNF水平不同:轻度组最低(8.2±0.4)ng/ml、重度组最高(9.5±0.3)ng/ml,差异有统计学意义(P0.05)。哮喘病情程度越重,血清BDNF的表达水平越高,两者呈正相关(r=0.756,P0.05)。3.哮喘组中33例具有特应性体质,27例无特应性体质,两者的血清BDNF水平分别为(9.1±0.6)ng/ml、(8.4±0.5)ng/ml,进行比较结果显示具有特应性体质的哮喘患儿血清BDNF的水平更高,差异有统计学意义(P0.05)。相关性分析结果显示两者呈正相关关系(r=0.504,P0.05)。结论:BDNF可能在儿童哮喘的发病机制中起着一定的作用,与患儿的特应性体质有关,并且其血清表达水平与哮喘病情严重程度相关,检测血清BDNF水平将有助于评估哮喘患儿的病情,对判断儿童哮喘的病情发展可能有一定的作用,为儿童哮喘的防治提供新思路。
[Abstract]:Objective: To observe the effect of brain-derived neurotrophic factor (BDNF) expression level in children with asthma and investigate the relationship between the possible role of BDNF in the pathogenesis of asthma in children with asthma severity and disease. Methods: 60 cases of asthma in children with acute asthma group from July 2015 to July 2016 in the First Affiliated Hospital of Shihezi University Medical Department of Pediatrics the hospital, of which 39 were male, 21 were female, aged 1.3~14.0 years old, the average (8.1 + 3.2) years; asthma in children according to the severity of asthma were divided into mild group, 18 cases, 25 cases of moderate group and severe group 17 cases; 60 cases of control group were selected in our hospital and 38 healthy children. Cases of male, female 22 cases, age 1.1~14.4 years old, the average (7.4 + 3.2) years old, and collected basic clinical data. In asthmatic children in acute stage respectively before treatment and symptom remission (no wheezing symptoms, lung without wheeze, be. Standardized control treatment) fasting venous blood 2ml, the control group to visit the same day fasting peripheral venous blood 2ml, room temperature (20~25 C) 60min 3000r/min centrifugal 10min, serum packed in EP tubes, -70 C refrigerator retained for inspection, to avoid repeated freezing and thawing. The application of enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) to detect the expression of BDNF in serum of two groups of children, to compare the differences among different severity of asthma children serum BDNF level, and analyze the relationship between BDNF level in children with asthma severity, and clinical base collected data for statistical analysis. The data using SPSS 17 statistical software measurement. Data to mean + standard deviation (? X + s) said that the normal distribution and homogeneity of variance using the t test or analysis of variance, homogeneity of variance using non parametric test; count data expressed as a ratio, chi square test Were compared; the correlation between variables by correlation analysis.P0.05 the difference was statistically significant. Results: the symptoms of acute attack group and remission group 1. asthma BDNF levels were (8.8 + 0.6) ng/ml, (6.4 + 0.9) ng/ml, were significantly higher than the control group (1.9 + 1.2) ng/ml, with acute exacerbation group was the highest (P0.05), remission of BDNF level is lower than the onset of illness (P0.05).2. in children with asthma severity, the serum level of BDNF in different groups: mild minimum (8.2 + 0.4) ng/ml, the highest in severe group (9.5 + 0.3) ng/ml, the difference was statistically significant (P0.05). Asthma severity, expression the serum level of BDNF is high. There is a positive correlation between them (r=0.756, P0.05).3. in the asthma group of 33 patients with atopy, 27 cases without atopy, serum BDNF levels of the two respectively (9.1 + 0.6) ng/ml, (8.4 + 0.5) ng/ ml, the results were compared with that of atopic children with asthma constitution The serum levels of BDNF were higher, the difference was statistically significant (P0.05). The results of correlation analysis showed that there was a positive correlation between (r=0.504, P0.05). Conclusion: plays a certain role in the pathogenesis of BDNF in childhood asthma, associated with atopy physique, and the expression level of serum and severe asthma the degree of correlation, serum BDNF level will help children with asthma severity assessment, to determine the development of asthma in children disease may have a certain effect, to provide new ideas for prevention and treatment of asthma in children.

【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.6

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