热休克蛋白70对新生大鼠缺氧性肺动脉高压的保护作用

发布时间：2018-03-01 14:30

本文关键词： 热休克蛋白缺氧诱导因子-α 缺氧性肺动脉高压新生大鼠　出处：《中国当代儿科杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨热休克蛋白70(HSP70)在新生大鼠缺氧性肺动脉高压(HPH)中的保护作用。方法将128只新生大鼠随机分为空白对照组、HPH模型组、空病毒对照组和HSP70组(n=32)。建立HPH模型前分别向空白对照组和HPH模型组大鼠通过尾静脉注射5μL无菌盐水,向空病毒对照组大鼠通过尾静脉注射5μL Ad-GFP(1 010 PFU/m L),向HSP70组大鼠通过尾静脉注射5μL Ad-HSP70(1 010 PFU/m L),转染后除空白对照组外,其余3组建立HPH模型。分别于建模后3、7、10、14 d,采用多导生理记录仪记录各组平均肺动脉压力(m PAP),光学及电子显微镜观察肺血管组织结构及重塑指标,Western blot法检测各组新生大鼠肺组织中HSP70、缺氧诱导因子-1α(HIF-1α)、缩血管因子内皮素-1(ET-1)、诱导型一氧化氮合酶(i NOS)蛋白表达水平。结果 HPH模型组与空病毒对照组在3、7、10、14 d时的m PAP水平高于空白对照组(P0.05)。缺氧7、10 d时,空白对照组及HSP70组MA%、MT%低于HPH模型组和空病毒对照组(P0.01);缺氧14 d时,HPH模型组、空病毒对照组和HSP70组MA%、MT%比较差异无统计学意义(P0.05),但高于空白对照组(P0.01)。缺氧3、7、10 d时,HSP70组HSP70的蛋白表达水平高于HPH模型组、空病毒对照组和空白对照组(P0.01);HSP70组HIF-1α、ET-1和i NOS表达水平低于HPH模型组和空病毒对照组(P0.05),而与空白对照组比较差异无统计学意义(P0.05)。结论转染HSP70可以提高HPH新生大鼠肺组织HSP70表达,下调HIF-1α、ET-1、i NOS表达,减轻肺血管重塑,降低肺动脉压力,可能成为治疗新生儿HPH的一种新策略。
[Abstract]:Objective to investigate the protective effect of heat shock protein 70 (HSP70) on hypoxic pulmonary hypertension (HPH) in neonatal rats. Before the establishment of HPH model, rats in blank control group and HPH model group were injected with 5 渭 L aseptic saline through tail vein, respectively. Rats in the empty virus group were injected with 5 渭 L Ad-GFP(1 010 PFU/m L Ad-GFP(1 through tail vein, and 5 渭 L Ad-HSP70(1 010 PFU/m L with 5 渭 L Ad-HSP70(1 010 PFU/m L in HSP70 group. After transfection, 5 渭 L Ad-HSP70(1 010 PFU/m L was injected into the control group except the blank control group. The HPH model was established in the other three groups, and the mean pulmonary artery pressure was recorded by multichannel physiological recorder on the 14th day after modeling. The tissue structure and remodeling index of pulmonary vessels were observed by optical and electron microscopy. The expression levels of HSP70, HIF-1 伪, endothelin-1, inducible nitric oxide synthase (iNOS) protein in lung tissue of newborn rats were higher than those in control group at day 14. Results the level of m PAP in HPH model group and empty virus control group was higher than that in blank control group at day 14. In the exposure group, P0.05, 10 days after hypoxia, The percentage of MT% in the blank control group and HSP70 group was lower than that in the HPH model group and the empty virus control group (P0.01). There was no significant difference in MT% between the control group and the HSP70 group, but it was higher than that in the blank control group (P 0.01). The expression of HSP70 protein in the HSP70 group was higher than that in the HPH model group at 10 days after hypoxia. The expression levels of HIF-1 伪 -ET-1 and I NOS in the empty virus control group and the blank control group were lower than those in the HPH model group and the empty virus control group, but there was no significant difference compared with the blank control group. Conclusion transfection of HSP70 can increase the expression of HSP70 in the lung tissue of the neonatal HPH rats. Down-regulating the expression of HIF-1 伪 -ET-1 NOS, reducing pulmonary vascular remodeling and reducing pulmonary artery pressure may be a new strategy for the treatment of neonatal HPH.
【作者单位】：新疆医科大学第一附属医院新生儿科;新疆伊犁州奎屯医院儿科;

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