新生大鼠缺氧缺血性脑损伤HIF-1α表达与细胞凋亡的关系及干预研究

发布时间：2018-03-02 17:12

本文选题：缺氧缺血性脑损伤　切入点：新生大鼠　出处：《中西医结合心脑血管病杂志》2012年06期 　论文类型：期刊论文

【摘要】：目的观察新生大鼠缺氧缺血性脑损伤(HIBD)后及2-甲氧基雌二醇(2ME2)干预后对脑组织缺氧诱导因子-1α(HIF-1α)表达趋势的变化与细胞凋亡的关系,探讨2ME2对HIBD的影响及可能的作用。方法将7d龄Wistar大鼠120只,随机分为假手术组、HIBD组及2ME2干预组。其中后两组又根据HIBD后处死动物的时间不同随机分为7个亚组。采用免疫组化的方法对各组新生大鼠脑组织HIF-1α和Caspase-3的表达进行检测及用TUNEL法测定细胞凋亡指数(AI)。结果 HIF-1α正常情况下低水平表达,HIBD后12h达高峰,后逐渐降低;Caspase-3表达于HIBD后6h明显升高,2d时达高峰;细胞凋亡指数于HIBD后3h升高,随着观察时间的推移,逐渐增多。2ME2干预后各时间点HIF-1α和Caspase-3的表达水平及细胞凋亡指数均较HIBD组明显降低,差异有统计学意义(P0.05)。结论 HIBD后HIF-1α表达增高,诱导细胞凋亡,促进了HIBD的进展;2ME2干预后,抑制了HIF-1α的表达,从而发挥脑保护作用。
[Abstract]:Objective to observe the relationship between the changes of HIF-1 伪 HIF-1 伪 expression and apoptosis in neonatal rats after hypoxic-ischemic brain injury (HIBD) and the intervention of 2-methoxyestradiol (2-methoxyestradiol) 2ME2). To investigate the effect of 2ME2 on HIBD and its possible effects methods 120 Wistar rats aged 7 days were selected. The latter two groups were randomly divided into 7 subgroups according to the time of death after HIBD. The expression of HIF-1 伪 and Caspase-3 in brain tissue of newborn rats in each group was detected by immunohistochemical method. Apoptosis index was measured by TUNEL method. Results the expression of HIF-1 伪 reached its peak 12 hours after HIBD. The expression of Caspase-3 increased significantly at 6 h after HIBD and reached the peak at 2 d after HIBD, and the apoptotic index increased at 3 h after HIBD, and increased with the time of observation. The expression level of HIF-1 伪 and Caspase-3 and the apoptosis index were significantly lower than those in HIBD group. Conclusion the expression of HIF-1 伪 increased after HIBD and induced apoptosis, which promoted the progression of HIBD. It inhibited the expression of HIF-1 伪, thus exerting the protective effect of brain.
【作者单位】：山西医科大学;山西医科大学第一临床医学院;
【基金】：山西省回国留学人员科技基金资助项目(No.2011-095) 山西省卫生厅科技攻关基金项目(No.2011018)
【分类号】：R722.1

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