香诃止泻方治疗脾虚泻临床疗效观察及实验研究

发布时间：2018-03-08 21:10

本文选题：香诃止泻方　切入点：小儿　出处：《云南中医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:泄泻为儿科常见病多发病,为儿科重点防治四病之一。香诃止泻方系导师熊磊用于治疗小儿脾虚泄泻的经验方,本研究对该方临床疗效、安全性及作用机制进行初步评价,为研制医院制剂提供前期工作基础。方法:1.临床研究:在云南省中医医院门诊收集符合脾虚泄泻纳入标准的60例患儿,采用香诃止泻方口服治疗6天,自身前后对照观察给药后患儿症状积分变化以及有效率。2.实验研究:通过单次给药毒性实验评价香诃止泻方安全性;通过小鼠胃排空及肠推进实验,对该方作用机制进行初步探讨。结果:1.临床研究:治疗后积分较治疗前明显降低,临床有效率达90%,对主要症状有效率达90%,次要症状有效率达83.3%。2.实验研究:香诃止泻方小鼠的日最大给药量为440.01g/kg(生药),约成年人(60kg)日临床用量(1.83g/kg)的240倍。小鼠胃排空实验及肠推进实验中,硫酸阿托品及香诃止泻方高剂量组能提高胃残留率和抑制肠推进,与正常对照组相比差异有统计学意义(P0.05)。结论:香诃止泻方对脾虚型泄泻治疗疗效确切,且对脾虚泄泻带来诸症腹泻次数、大便性状、病程、呕吐、腹痛、口渴、纳差等均有明显改善作用。通过实验结果推测药物止泻作用机制可能与抑制胃肠蠕动有关。本研究为临床应用香诃止泻方治疗脾虚型泄泻提供了依据。
[Abstract]:Objective: diarrhea is one of the most common diseases in pediatrics and one of the most important four diseases in pediatrics. Xiong Lei, a tutor of XianghaZhixie recipe, was used to treat diarrhea due to spleen deficiency in children. Methods: 1. Clinical study: 60 children who met the criteria of diarrhea due to spleen deficiency were collected from outpatient clinic of Yunnan Provincial traditional Chinese Medicine Hospital. After 6 days of oral treatment with Xianghazhixie recipe, the changes of symptom score and effective rate were observed before and after administration. 2. Experimental study: the safety of Xianghe Zhixie recipe was evaluated by single dose toxicity experiment; The action mechanism of this prescription was preliminarily discussed by gastric emptying and intestinal propulsion test in mice. Results: 1. Clinical study: the score after treatment was significantly lower than that before treatment. The clinical effective rate was 90, the effective rate for main symptoms was 90, and the effective rate for secondary symptoms was 83.3. 2. Experimental study: the maximum daily dose of Xianghazhixie recipe in mice was 440.01g / kg. The gastric emptying of mice was 240 times as much as the daily clinical dosage of 1.83 g / kg. In experiments and bowel propulsion experiments, The high dose group of atropine sulfate and Xianghazhixie prescription could increase the gastric residual rate and inhibit the intestinal propulsion, and the difference was statistically significant compared with the normal control group (P 0.05). Conclusion: Xianghazhixie recipe is effective in treating diarrhea of spleen deficiency type. And the number of diarrhea, defecation traits, course of disease, vomiting, abdominal pain, thirst caused by diarrhea due to spleen deficiency, According to the experimental results, the mechanism of antidiarrhea may be related to the inhibition of gastrointestinal peristalsis. This study provides a basis for the clinical application of XianghaZhixie recipe in the treatment of diarrhea due to spleen deficiency.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R272

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