卵清蛋白致敏幼鼠血IL-4、IFN-γ检测及意义

发布时间：2018-03-16 13:27

本文选题：食物过敏　切入点：卵清蛋白　出处：《福建医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：通过观察3周龄SD幼鼠食物过敏(FA)动物模型血IL-4、IFN-γ含量及IL-4/IFN-γ比值在致敏前、致敏激发后变化，探讨TH1、TH2型细胞的活化状态在FA发生中的作用及其意义。方法：选取16只3周龄清洁级断乳雌性SD幼鼠作为实验对象，随机分为食物过敏组(FA组)和对照组(NS组)，每组8只。通过低剂量卵白蛋白(OVA)腹腔注射基础致敏及高剂量OVA灌胃激发建立SD幼鼠OVA食物过敏(FA)。观察两组幼鼠致敏前、致敏激发后体重及活动等情况、血OVA-IgE及肠黏膜组织变化进行幼鼠FA模型评价。采用ELISA法连续检测致敏激发前后两组幼鼠血IL-4、IFN-γ含量。采用SPSS13.0软件包进行统计分析，α=0.05为显著性检验标准。结果：FA组幼鼠出现不同程度竖毛，毛发失去光泽，活动减少或俯卧不动，抓耳挠腮或躁动不安及排不成形稀便，与NS组相比，FA组幼鼠末次激发后体重有减轻趋势；FA组幼鼠肠道黏膜有较多炎性细胞和嗜酸性粒细胞浸润；致敏激发后FA组幼鼠血OVA-IgE含量显著升高，与NS组比较差别有统计学意义(P＜0.01)。FA组幼鼠致敏前、致敏激发后血IL-4含量(ng/L)分别为36.375±4.312、71.075±10.055，NS组分别为35.394±3.635、41.590±6.757，致敏前两组幼鼠血IL-4含量比较无显著差别，致敏激发后FA组IL-4含量明显升高，与NS组比较差别有统计学意义(P＜0.01)；FA组幼鼠致敏前、致敏激发后血IFN-γ含量(ng/L)分别为100.849±9.052、259.146±54.008，NS组分别为100.164±18.496、115.654±18.428，，致敏前两组幼鼠血IFN-γ含量比较无显著差别，激发后FA组IFN-γ含量明显升高，与NS组比较差别有统计学意义(P＜0.01)；随着致敏后激发次数的增多，FA组幼鼠血IL-4、IFN-γ浓度逐渐升高，到末次激发后达到高峰，血IL-4、IFN-γ浓度每一次激发后同一时间FA组SD幼鼠均较NS组有明显的升高；两组幼鼠血清IL-4/IFN-γ比值在致敏前及致敏激发后比较均无显著差别(P＞0.05)。结论：采用OVA腹腔注射基础致敏，结合OVA灌胃激发可建立幼鼠FA动物模型；.本课题所建立的FA模型幼鼠TH2及TH1细胞都处于高功能状态，可能存在IgE介导的体液免疫反应及细胞介导的迟发型变态反应。
[Abstract]:Objective: to observe the changes of serum IL-4 / IFN- 纬 content and IL-4 / IFN- 纬 ratio before and after sensitization in a 3-week-old SD rat model of food allergy (FAA), and to explore the role and significance of activation of TH1 / TH2 type cells in the pathogenesis of FA. Methods: sixteen 3-week-old female SD rats with cleanness grade weaning were selected as experimental objects. The rats were randomly divided into two groups: food allergy group (FA group) and control group (NS group, 8 rats in each group). OVA food allergy was induced by intraperitoneal injection of low dose ovalbumin (OVA) and high dose OVA intragastrically. The changes of serum OVA-IgE and intestinal mucosal tissue were evaluated by FA model of young rats. The serum IL-4 and IFN- 纬 levels were continuously detected by ELISA method before and after sensitization. SPSS13.0 software package was used for statistical analysis, 伪 -0. 05 was the significant test standard. Results in the control group, different degrees of hair were observed, the hair lost luster, the activity decreased or the body did not move, the ears scratched the cheek or restless, and the defecation was out of shape. Compared with NS group, the body weight of the young rats in FA group showed a tendency to lose weight after the last stimulation, and there were more inflammatory cells and eosinophil infiltration in intestinal mucosa in FA group, and the serum OVA-IgE content in FA group increased significantly after sensitization and stimulation. There was significant difference between NS group and NS group (P < 0.01). Before sensitization and after sensitization, the serum IL-4 content was 36.375 卤4.312 ~ 71.075 卤10.055 卤10.055N, respectively. There was no significant difference in serum IL-4 content between the two groups before and after sensitization. The IL-4 content of FA group was significantly increased after sensitizing and arousing, and that of NS group was 35.394 卤3.635U, 41.590 卤6.757, respectively. There was significant difference between NS group and NS group (P < 0.01). Before and after sensitization, the IFN- 纬 content of serum IFN- 纬 was 100.849 卤9.052 卤54.008NS group (100.164 卤18.496115.654 卤18.428) respectively. Compared with NS group, the difference was significant (P < 0.01), and the serum IL-4 IFN- 纬 concentration in FA group gradually increased with the increase of sensitizing times, and reached the peak after the last stimulation. The serum IL-4 / IFN- 纬 ratio in FA group was significantly higher than that in NS group at the same time after each stimulation, and there was no significant difference in serum IL-4 / IFN- 纬 ratio between the two groups before and after sensitization (P > 0.05). Conclusion: the FA animal model of young rats can be established by intraperitoneal injection of OVA, combined with the stimulation of OVA. The TH2 and TH1 cells of the model of FA in this study are all in high functional state. There may be humoral immune response mediated by IgE and delayed hypersensitivity mediated by cells.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.9

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