2014、2015年度武汉地区急性呼吸道感染患儿鼻咽分泌物RSV和hMPV基因检测分析

发布时间：2018-03-17 15:34

  本文选题：呼吸道感染　切入点：呼吸道合胞病毒　出处：《山东医药》2017年03期 　论文类型：期刊论文

【摘要】：目的观察2014年度和2015年度武汉地区急性呼吸道感染(ARTIs)患儿鼻咽分泌物中呼吸道合胞病毒(RSV)、人偏肺病毒(h MPV)基因检出情况,并分析RSV、h MPV感染的分布特点。方法 2014年1月~2015年12月收治的0~14岁的ARTIs患儿816例(2014年度456例、2015年度360例),采集患儿鼻咽分泌物,采用RTPCR检测RSV G基因、h MPV F基因,通过DNA测序分析基因型。观察RSV、h MPV感染在不同年度、季节及不同性别、年龄、病种患儿中的分布情况。结果 816例患儿中,检出RSV 187例,其中2014年度109例、2015年度78例;检出h MPV 116例,其中2014年度69例、2015年度47例;2014年度和2015年度h MPV、RSV的检出率差异均无统计学意义。1~2岁年龄组患儿h MPV检出率最高,5岁以内患儿h MPV检出率高于90%;5岁以内患儿RSV检出率也较高,高于90%。喘息性支气管炎、毛细支气管炎患儿h MPV检出率显著高于其他感染部位(P均0.05),上呼吸道感染、支气管炎、支气管肺炎患儿h MPV检出率较低(10%左右);支气管肺炎、毛细支气管炎患儿RSV检出率高于其他感染部位(P均0.05),喘息性支气管炎患儿RSV检出率最低。每年2~3月h MPV、RSV检出率最高,6~9月最低。基因型分析显示,2014、2015年度患儿检出RSV基因型均以A型为主,2014年度患儿检出h MPV基因型以A2型为主、2015年度以B型为主。结论 RSV和h MPV是武汉地区ARTIs患儿常见的病原体,RSV、h MPV感染多发生在5岁以下患儿,且多发于冬季和早春;RSV感染与支气管肺炎、毛细支气管炎有关,h MPV感染与喘息性支气管炎、毛细支气管炎有关;RSV流行毒株以A型为主,h MPV流行毒株在2014、2015年度存在差异。
[Abstract]:Objective to observe the detection of respiratory syncytial virus (RSVV) and human metapulmonary virus (hMPV) gene in nasopharyngeal secretions of children with ART Isa in Wuhan in 2014 and 2015. Methods from January 2014 to December 2015, 816 patients with ARTIs aged from 0 to 14 years old and 456 cases with ARTIs from 2014 to 2015 were collected and their nasopharyngeal secretions were collected. RTPCR was used to detect h MPV F gene of RSV G gene. Genotypes were analyzed by DNA sequencing. The distribution of RSVH MPV infection in different year, season, sex, age and disease was observed. Results 187 cases of RSV were detected in 816 cases, including 109 cases in 2014 and 78 cases in 2015. H MPV was detected in 116 cases. There was no significant difference in the detection rate of h MPV between the 47 cases in 2014 and 2015. The detection rate of h MPV in the 2 year old group was the highest. The detection rate of h MPV in children under 5 years old was higher than that in 90 years old children under 5 years old, and the detection rate of RSV was also higher in the age group of 2 years old than that in the age group of 90 years old. The detection rate of h MPV in children with asthmatic bronchitis and bronchiolitis was significantly higher than that in other infection sites (P 0.05). The detection rate of MPV in children with upper respiratory tract infection, bronchitis and bronchopneumonia was about 10%. The detection rate of RSV in children with bronchiolitis was higher than that in other infection sites (P < 0.05), and the detection rate of RSV in asthmatic bronchitis was the lowest. The detection rate of RSV was the highest in children with bronchiolitis from 2 h to March every year and the lowest from 6 to September. Genotype analysis showed that RSV was detected in children with bronchiolitis in 20142015. The h MPV genotypes were mainly detected by type A in 2014 and B in 2015. Conclusion RSV and h MPV are the common pathogens of ARTIs in Wuhan area, and most of them occur in children under 5 years of age. Most of RSV infection in winter and early spring were associated with bronchopneumonia, bronchiolitis related MPV infection and wheezing bronchitis, and bronchiolitis related serotype A was the main epidemic strain of MPV.
【作者单位】： 湖北省武汉市普仁医院;
【分类号】：R725.6
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本文编号：1625356