儿童哮喘患者中医证型的代谢组学研究

发布时间：2018-03-27 17:06

本文选题：代谢组学　切入点：肺脾气虚　出处：《中华中医药学刊》2017年01期

【摘要】：目的:基于气相色谱/质谱(GC/MS)技术的代谢组学方法,分析儿童哮喘两种中医证型患者尿液中的内源性小分子代谢物,探索两种中医证型分类的物质基础。方法:采用GC/MS技术检测儿童哮喘肺脾气虚型、气阴两虚型患者以及正常儿童的尿液中小分子代谢物,利用SIMCA-P软件进行主成分分析(principal component analysis,PCA)及正交偏最小二乘法(OPLSA)分析,研究尿液中小分子代谢物在各组间差异。结果:儿童哮喘肺脾气虚型、气阴两虚型患者代谢谱具有良好的分离趋势,并与正常儿童对照组有明显区分。利用代谢物谱库(如Wiley和NIST质谱库)及本实验室建立的标准品代谢物谱库鉴定了17个差异代谢物。儿童哮喘两种"证型"患者间尿液中丁酸、磷酸盐、L-苏氨酸、肌酐、甘氨酸、D-葡萄糖、马尿酸、假尿苷的相对含量存在显著差异,这些代谢物与能量代谢、肠道菌群代谢、氨基酸代谢和核苷酸代谢异常相关,它们是区分这两种中医证型的潜在生物标志物。结论:代谢组学技术能够全面地反映肺脾气虚型、气阴两虚型儿童哮喘的代谢共性和各自特征,代谢组学所研究的体内小分子代谢物可能是中医分型的物质基础。
[Abstract]:Objective: to analyze the endogenous small molecule metabolites in urine of two TCM syndrome types of asthma in children based on GC / MS. Methods: GC/MS technique was used to detect small molecule metabolites in urine of asthmatic children with deficiency of lung and spleen qi, deficiency of both qi and yin, and normal children. Principal component analysis (PCA) and orthogonal partial least square method (OPLSA) were used to study the differences of small molecular metabolites in urine among groups by SIMCA-P software. Results: the deficiency of lung and spleen qi in asthmatic children was studied. The metabolic spectrum of patients with deficiency of Qi and Yin has a good separation trend. 17 different metabolites were identified by using the metabolite spectrum library (such as Wiley and NIST mass spectrometry) and the standard metabolite library established in our laboratory. The butyric acid in urine was identified between the two "syndrome types" of childhood asthma. There were significant differences in the relative contents of phosphate-L-threonine, creatinine, glycine D-glucose, hippuric acid and pseudouridine. These metabolites were correlated with abnormal energy metabolism, intestinal microbial metabolism, amino acid metabolism and nucleotide metabolism. They are potential biomarkers for differentiating these two types of TCM syndromes. Conclusion: metabolic techniques can fully reflect the metabolic commonness and characteristics of asthmatic children with deficiency of lung and spleen qi and yin. The metabolites of small molecules in vivo may be the material basis of TCM classification.
【作者单位】：上海市宝山区中西医结合医院药剂科;杭州师范大学医学院;上海中医药大学附属曙光医院宝山分院;
【基金】：国家自然科学基金项目(81473475) 浙江省自然科学基金项目(LY16H270018) 上海市进一步加快中医药事业发展三年行动计划(2014-2016年)项目(ZY3-RCPY-3-1013) 上海市宝山区科技创新专项基金项目(12-E-20)
【分类号】：R272

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