右美托咪定对缺血缺氧性脑损伤新生大鼠的神经保护作用及机制

发布时间：2018-04-04 18:20

本文选题：右美托咪定　切入点：缺血缺氧性脑损伤　出处：《中国老年学杂志》2017年10期

【摘要】：目的探讨右美托咪定(DEX)对缺血缺氧性脑损伤(HIBD)新生大鼠神经功能的影响。方法 36只健康雄性SD大鼠,按照随机数字法分为假手术组(S组)、模型组(M组)和DEX组(D组),每组12只。D组大鼠腹腔注射100μg/kg盐酸右美托咪定注射液,而S组和M组大鼠按照相同的剂量注射等量的生理盐水。每组随机取8只大鼠进行神经功能缺损程度评分(NDS),应用酶联免疫吸附测定法检测脑组织匀浆液中的炎症因子白细胞介素(IL)-6、IL-8、IL-10和肿瘤坏死因子(TNF)-α,采用分光光度法测量谷氨酸(Glu)和γ-氨基丁酸(GABA)水平,采用氯化三苯基四氮唑(TTC)染色法计算大脑的梗死体积和脑梗死体积百分比。结果与S组相比,M组和D组NDS评分及脑梗死相关指标均明显升高,且D组各指标明显低于M组(P0.05);M组和D组脑组织匀浆中IL-6、IL-8和TNF-α水平明显高于S组,而IL-10水平显著低于S组,且D组炎症因子水平改善情况明显优于M组(P0.05);与D组相比,其他两组Glu水平明显升高,而GABA水平显著下降,另外,与M组相比,D组脑组织中氨基酸水平的改善情况更明显(均P0.05)。结论 DEX可以改善HIBD新生大鼠的神经功能,减少脑梗体积和脑梗死体积百分比,降低脑组织中的炎症因子和兴奋性氨基酸的水平,从而发挥神经保护功能,为临床用药提供理论依据。
[Abstract]:Objective to investigate the effect of dexmetomidine (DEX) on neural function in neonatal rats with ischemic hypoxic brain injury (HIBD).Methods Thirty-six healthy male Sprague-Dawley rats were randomly divided into sham-operated group (group S, model group M) and DEX group (group D, n = 12). Each group (n = 12) was intraperitoneally injected with dexmetidine hydrochloride (100 渭 g/kg).Rats in group S and group M received the same amount of saline at the same dose.Eight rats in each group were randomly selected to evaluate the degree of neurological impairment. The inflammatory cytokines IL-10 and TNF- 伪 in the brain homogenate were detected by enzyme-linked immunosorbent assay (Elisa). The levels of TNF- 伪 and IL-10 in the brain homogenate were measured by spectrophotometry.The levels of glutamate Gluand 纬 -aminobutyric acid GABA,The infarct volume and the percentage of cerebral infarct volume were calculated by TTC staining.Results compared with the S group, the NDS scores and the cerebral infarction related indexes in the M group and D group were significantly increased, and the levels of IL-6 IL-8 and TNF- 伪 in the brain homogenate of the M group and D group were significantly lower than those in the S group, and the level of IL-10 was significantly lower in the D group than that in the S group, and the levels of IL-6 IL-8 and TNF- 伪 in the brain homogenate of the M group were significantly lower than those in the S group.The level of inflammatory cytokines in group D was significantly better than that in group M (P 0.05), the level of Glu in the other two groups was significantly higher than that in group D, and the level of GABA was significantly decreased. In addition, the improvement of amino acid level in brain tissue of group D was more obvious than that in group M (all P 0.05).Conclusion DEX can improve the neural function, reduce the volume of cerebral infarction and the percentage of cerebral infarction volume, decrease the levels of inflammatory factors and excitatory amino acids in brain tissue, and thus play a neuroprotective role in neonatal rats with HIBD.To provide theoretical basis for clinical use of drugs.
【作者单位】：山东省医学科学院附属医院麻醉科;济南市槐荫人民医院内科;山东省交通医院麻醉科;
【分类号】：R722.1

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