EV71型手足口病患儿外周血树突状细胞及细胞因子产生的调节机制研究

发布时间：2018-04-05 22:24

本文选题：肠道病毒EV　切入点：树突状细胞　出处：《安徽医科大学学报》2017年01期

【摘要】：目的探讨肠道病毒EV71型感染对树突状细胞(DC)成熟凋亡、DC中信号传导通路激活、细胞因子释放的作用机制。方法收集肠道病毒EV71型感染患儿40例,分为20例轻症型组及20例重症型组,正常对照组儿童20例。流式细胞仪检测各组儿童外周血DC表面标志分子CD11c、CD86、CD80和CD83表达的百分比;采用Western blot法检测DC中MAPK信号传导通路分子磷酸化水平;ELISA法检测同期血清中细胞因子的水平。结果随着EV71感染程度的加深,外周血DC中标志着细胞成熟的表面标志分子水平均有增加,CD83及CD80尤为显著;MAPK信号传导通路的活化程度逐渐加强;细胞因子水平的表达显著升高。结论 EV71感染可促进树突状细胞成熟凋亡、激活MAPK信号传导通路并增加细胞因子的释放,MAPK信号传导通路在EV71感染的DC中发挥显著调控作用。
[Abstract]:Objective to investigate the mechanism of signal transduction pathway activation and cytokine release in dendritic cell (DC) mature apoptotic DC infected with enterovirus type EV71.Methods 40 children with enterovirus EV71 type infection were divided into two groups: 20 cases of mild type and 20 cases of severe type, and 20 cases of normal control group.Flow cytometry was used to detect the percentage of CD11cU CD86T CD80 and CD83, and Western blot method was used to detect the phosphorylation level of MAPK signal transduction pathway molecules. Elisa was used to detect the level of cytokines in serum at the same time.Results with the deepening of EV71 infection, the activation of CD83 and CD80 signal transduction pathway was increased, and the expression of cytokines was significantly increased in peripheral blood DC.Conclusion EV71 infection can promote the maturation and apoptosis of dendritic cells, activate the MAPK signaling pathway and increase the release of cytokines. It plays a significant role in the regulation of DC infected with EV71.
【作者单位】：安徽医科大学第一附属医院检验科;安徽医科大学阜阳传染病临床学院阜阳市第二人民医院检验科;安徽医科大学阜阳传染病临床学院阜阳市第二人民医院感染科;
【基金】：安徽高校省级自然科学研究项目(编号:KJ2011A181)
【分类号】：R725.1

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