血清补体、免疫球蛋白及炎性细胞因子动态检测在儿童肺炎支原体感染中的应用价值

发布时间：2018-04-09 20:00

本文选题：肺炎支原体　切入点：儿童　出处：《中国免疫学杂志》2017年06期

【摘要】：目的:探讨动态检测血清补体、免疫球蛋白及炎性细胞因子在儿童肺炎支原体(MP)感染中的临床价值。方法:选择MP感染患儿60例作为MPP组,同期健康体检儿童60例作为对照组。采用免疫散射比浊法(INA)检测血清补体(C3、C4)及免疫球蛋白(IgA、IgG、IgM),采用酶联免疫吸附(ELISA)测定IL-8、IL-10、IL-13及TNF-α。结果:(1)MPP组急性期IgM、C3、C4、IL-8、TNF-α及IL-13显著高于恢复期及对照组(P0.05),且恢复期IL-8、TNF-α、IL-13仍显著高于对照组(P0.05);急性期IgA、IL-10显著低于对照组,且恢复期仍显著低于对照组(P0.05);急性期IgG与对照组无明显差异(P0.05),而恢复期显著高于急性期及对照组(P0.05);(2)MPP组重型IgM、IgG、C3、C4、IL-8、TNF-α及IL-13显著高于轻型及对照组,且轻型的IgM、IL-8、TNF-α、IL-13仍显著高于对照组(P0.05);重型组的IgA及IL-10显著低于轻型及对照组,且轻型仍显著低于对照组(P0.05)。结论:儿童MP感染具有明显的细胞及体液免疫功能紊乱,动态检测血清补体、免疫球蛋白及炎性细胞因子对评定疾病分期、病情程度具有重要价值。
[Abstract]:Objective: to investigate the clinical value of dynamic detection of serum complement, immunoglobulin and inflammatory cytokines in children with MPP infection.Methods: 60 children with MP infection were selected as MPP group and 60 healthy children as control group.The serum complement C _ (3) C _ (4) and immunoglobulin IgA (IgA) were detected by immunosensor turbidimetric assay (Ina), and IL-8 (IL-10) IL-13 and TNF- 伪 were determined by Elisa (ELISAs). The serum levels of IL-8 and TNF- 伪 were determined by enzyme-linked immunosorbent assay (Elisa).Conclusion: MP infection in children has obvious cellular and humoral immune dysfunction. Dynamic detection of serum complement, immunoglobulin and inflammatory cytokines is of great value in assessing the stage and severity of the disease.
【作者单位】：湖北省天门市第一人民医院检验科;
【分类号】：R446.6;R725.6

【相似文献】