广州地区人巨细胞病毒临床低传代株UL141基因的序列特征

发布时间：2018-04-13 14:40

本文选题：人巨细胞病毒 + 生物信息学　；参考：《医学研究生学报》2017年10期

【摘要】：目的 UL141基因的结构特征及多态性对发现人巨细胞病毒(HCMV)感染致病性机制具有指导意义。文中旨在研究广州地区HCMV临床低传代株UL141基因的序列特征。方法选取2016年3月至2017年3月在广州医科大学附属广东省妇儿医院儿科就诊的10例HCMV患儿,收集患儿尿液标本,从中分离低传代临床病毒株,应用多重PCR方法鉴定,对其进行HCMV UL141基因全序列扩增、克隆、鉴定、测序,并在Gen Bank搜索HCMV UL141同源序列,行序列分析。结果UL141基因存在UL141a及UL141b 2处开放阅读框架(ORF),分别由315及1017个核苷酸构成,Gen Bank登记号分别为DQl80372和DQl80371。UL141基因全长1277 bp,编码蛋白由338个氨基酸残基组成,UL141a及UL141b开放阅读框架ORF分别为315、1017 bp,其DNA序列比较保守,存在46处位点变异,变异皆为碱基替换,无插入或缺失。HCMV UL141编码蛋白翻译后修饰位点在所有分离株中均比较保守,所有分离株UL141蛋白的等电点均在8.36~8.68之间。结论广州地区HCMV临床低传代分离株UL141基因及其编码的氨基酸序列比较保守,但仍存在一定的多态性,为进一步揭示ULl41蛋白的功能及HCMV感染的致病机制奠定了基础。
[Abstract]:Objective to explore the pathogenicity of human cytomegalovirus (HCMV) infection by structural characteristics and polymorphism of UL141 gene.The purpose of this study was to study the sequence characteristics of UL141 gene of HCMV clinical low passage strain in Guangzhou.Methods Ten children with HCMV were selected from March 2016 to March 2017 in Guangdong Provincial Hospital for Women and Children of Guangzhou Medical University. Urine samples were collected and low passage clinical virus strains were isolated and identified by multiple PCR method.The whole sequence of HCMV UL141 gene was amplified, cloned, identified and sequenced. The homologous sequence of HCMV UL141 was searched by Gen Bank, and the sequence analysis was carried out.The ORF of the frame was 315 ~ 1017 BP, and its DNA sequence was conserved.There were 46 loci of mutation, all of which were base substitution. The post-translational modification sites of HCMV-encoding protein were conserved in all isolates. The isoelectric point of UL141 protein of all isolates was between 8.36 and 8.68.Conclusion the UL141 gene and its encoded amino acid sequence of the low passage HCMV isolates in Guangzhou are conserved, but there are still some polymorphisms, which lays a foundation for further revealing the function of ULl41 protein and the pathogenesis of HCMV infection.
【作者单位】：广州医科大学附属广东省妇儿医院/广东省妇幼保健院儿科;
【基金】：国家自然科学基金(81070516) 广东省自然科学基金(9151008901000085、2015A030313726、2016A030313788) 广东省医学科研基金(A2015133)
【分类号】：R725.1

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