基于GC-MS代谢组学的RSV肺炎小鼠尿液和粪便生物标记物研究

发布时间：2018-04-20 09:22

本文选题：气相色谱-质谱联用 + 尿液　；参考：《南京中医药大学学报》2017年02期

【摘要】：目的通过气相色谱-质谱联用(GC-MS)技术分析RSV肺炎小鼠尿液和粪便中代谢物的相对含量变化,寻找可协助RSV肺炎诊断的生物标记物。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,分别收集小鼠的尿液和粪便,经肟化和衍生化后,运用GC-MS检测尿液和粪便中所含有的代谢物及其相对含量的变化。结果 RSV肺炎小鼠的尿液中共测得38种代谢产物,其中乳酸、丙酸、L-脯氨酸为差异代谢产物(P0.05);粪便中共测得43种代谢物,其中亮氨酸、天冬氨酸、胱氨酸、酪氨酸、嘧啶有显著差异(P0.05)。结论运用GC-MS代谢组学技术寻找RSV肺炎小鼠尿液和粪便中的生物标记物具有可行性。
[Abstract]:Objective to analyze the relative contents of metabolites in urine and feces of RSV pneumonia mice by gas chromatography-mass spectrometry (GC-MS), and to search for biomarkers to assist the diagnosis of RSV pneumonia. Methods the RSV pneumonia model was established in BALB/c mice infected with RSV nasal drip. Urine and feces were collected respectively. After oximation and derivation, the metabolites and their relative contents in urine and feces were detected by GC-MS. Results A total of 38 metabolites were detected in urine of RSV pneumonia mice, among which lactic acid and L-proline were different metabolites (P 0.05), and 43 metabolites were determined in feces, including leucine, aspartic acid, cystine, tyrosine, leucine, aspartic acid, cystine, tyrosine, leucine, aspartic acid, cystine, tyrosine. There was significant difference in pyrimidine (P 0.05). Conclusion it is feasible to find the biomarkers in urine and feces of RSV pneumonia mice by using GC-MS metabolomics technique.
【作者单位】：江苏省儿童呼吸疾病(中医药)重点实验室南京中医药大学中医儿科学研究所;南京中医药大学医学代谢组学中心;
【基金】：江苏省自然科学基金(BK20161573) 江苏省高校自然科学研究重大项目(16KJA360002) 江苏高校优势学科建设工程资助项目
【分类号】：R725.6

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