卡波西样血管内皮瘤伴卡梅现象患儿GRa、GRβ的表达与激素敏感性的关系

发布时间：2018-04-23 20:10

本文选题：卡波西样血管内皮瘤 + 卡梅现象　；参考：《郑州大学》2017年硕士论文

【摘要】：目的探讨卡波西样血管内皮瘤(KHE)伴卡梅现象(KMP)患儿糖皮质激素受体(GRa、GRβ)的表达与激素敏感性的关系。方法收集自2013年5月年-2016年5月来我院治疗的卡波西样血管内皮瘤伴卡梅现象的患儿25例,男性9例,女性16例,平均年龄为(2.4±1.5)月。所有通过入选标准的患儿均进行为期1周的激素治疗,根据疗效评判标准,分为敏感组和抵抗组。待患儿的血小板得到提升,凝血功能得到改善,对于手术和麻醉的耐受力得到提高,并完善其他的术前准备,行手术治疗,以外科手术切除的15例多余的健康组织作为对照组,对其手术切除组织进行免疫组化SP法检测,比较分析敏感组、抵抗组、对照组糖皮质激素受体(GRa、GRβ)阳性表达的数量、强度和分布特点。结果1.GRa的表达强度在三组中的无统计学意义。GRa阳性细胞数量在敏感组中高于抵抗组(P0.05)。2.GRβ的表达强度在抵抗组中显著高于敏感组、对照组(P0.01);GRβ阳性细胞数量在敏感组、对照组、抵抗组中呈逐渐上升趋势,且有明显显著性意义(P0.01)。3.GRa/GRβ表达强度的比值在抵抗组中显著低于敏感组、对照组(P0.01);GRa/GRβ阳性细胞数量的比值在敏感组、对照组、抵抗组中呈逐渐下降趋势,且有明显显著性意义(P0.01)。结论1.KHE伴KMP患儿瘤体组织中GRa、GRβ的表达与GC敏感性密切相关。2.GC的敏感性可能与GRa有一定的关系,但相对GRβ来说不起主导作用。3.GRβ表达增高可能是GC抵抗的重要原因,并对GC敏感性的影响起主要作用。4.GRβ表达的增高可能对GRa起负性调节作用,GRa、GRβ表达比例的平衡失调,可能是GC抵抗的重要原因,还有待进一步研究证实。
[Abstract]:Objective to investigate the relationship between the expression of glucocorticoid receptor (GRAR 尾) and hormone sensitivity in children with carpoxiform hemangioendothelioma (KHEA) and KMPs. Methods from May 2013 to May 2016, 25 cases of Carbosiform hemangioendothelioma with Kamei phenomenon were collected, including 9 males and 16 females, with an average age of 2.4 卤1.5 months. All the children who passed the inclusion criteria were treated with hormone for one week and were divided into sensitive group and resistance group according to the criteria of curative effect. When the platelets were improved, the coagulation function was improved, the tolerance to surgery and anesthesia was improved, and other preoperative preparations were improved, surgical treatment was performed, and 15 cases of superfluous healthy tissue surgically resected were used as the control group. Immunohistochemical SP method was used to detect the expression of glucocorticoid receptor GRaR 尾 in sensitive group, resistance group and control group. Results the expression intensity of 1.GRa in the three groups was significantly higher than that in the resistant group. The expression of GRa 尾 was significantly higher in the sensitive group than in the resistant group, and the number of positive cells in the control group was higher than that in the sensitive group and the control group, and the number of GRa 尾 positive cells in the control group was significantly higher than that in the resistant group, and the number of GRa 尾 positive cells in the control group was significantly higher than that in the control group. In the resistance group, the ratio of GRAR 尾 expression was significantly lower than that in the sensitive group, and the ratio of the number of GRAR 尾 positive cells in the control group was decreased gradually in the sensitive group, and in the control group and resistance group, there was a decrease in the number of GRAR 尾 positive cells in the control group and in the control group, while in the control group, the ratio of GRaR 尾 positive cells in the control group was decreased gradually. And there was significant difference in P0.01C. Conclusion the expression of gr 尾 in the tumor tissues of children with 1.KHE and KMP is closely related to the sensitivity of GC. 2. The sensitivity of GC may be related to the sensitivity of GC, but the increase of expression of gr 尾 may be an important reason for GC resistance, but it does not play a leading role in the expression of gr 尾. 4. The increased expression of gr 尾 may play a negative role in regulating the balance of gr 尾 expression in GRa and may be an important reason for GC resistance.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R732.2

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