呼吸道合胞病毒毛细支气管炎患儿外周血TGF-β和IL-10的含量及意义

发布时间：2018-04-25 00:22

本文选题：转化生长因子-β + 白细胞介素-10　；参考：《山东大学》2012年硕士论文

【摘要】：目的：毛细支气管炎是婴幼儿最常见的肺间质性炎症,在毛细支气管炎病原体中,呼吸道合胞病毒最常见,占70%以上。炎性因子介导的炎症反应在毛细支气管炎的发病机制中有重要作用。本实验检测呼吸道合胞病毒毛细支气管炎患儿外周血中转化生长因子-β(TGF-β)和白细胞介素-10(IL-10)浓度的变化,并探讨其临床意义。研究对象和方法：选择自2011年10月至2012年4月在聊城市人民医院住院的呼吸道合胞病毒毛细支气管炎患儿80例(咽拭子呼吸道合胞病毒-PCR阳性者)作为研究对象,根据是否合并心力衰竭,分为毛细支气管炎组(简称普通组)和毛细支气管炎组合并心衰组(简称心衰组),40例健康儿童作为对照组。应用ELISA双抗体夹心法检测各组儿童外周血TGF-β和IL-10的含量。结果： 1.治疗前,普通组外周血TGF-β含量(21.77±4.47pg/ml)和心衰组外周血TGF-β含量(14.86±3.72pg/ml),均明显低于对照组(53.78±4.10pg/ml)(p均0.05)。 2.治疗前,心衰组外周血TGF-β水平(14.86±3.72pg/ml)明显低于普通组(21.77±4.47pg/ml)(p0.05)。 3.治疗前,普通组外周血IL-10含量(17.55±2.47pg/ml)、心衰组外周血IL-10含量(10.80±2.72pg/ml),均明显低于对照组(47.52±4.10pg/ml)(p均0.05)。 4.治疗前,心衰组外周血IL-10水平(10.80±2.72pg/m1)明显低于普通组(17.55±2.47pg/m1)(p0.05)。 5.治疗后,普通组外周血TGF-β含量(46.21±5.77pg/m1)较治疗前(21.77±4.47pg/m1)明显增高(p均0.05)。心衰组外周血TGF-β含量(35.68±4.03pg/m1)亦较治疗前(14.86±3.72pg/m1)明显增高(p均0.05)。 6.治疗后,心衰组外周血TGF-β水平(35.68±4.03pg/m1)仍低于普通组(46.21±5.77pg/m1)(p均0.05)。 7.治疗后,普通组外周血IL-10含量(30.50±2.77pg/m1)较治疗前(17.55±2.47pg/m1)明显增高(p均0.05),心衰组外周血IL-10含量(21.93±3.03pg/m1)亦较治疗前(10.80±2.72pg/m1)明显增高(p均0.05)。 8.治疗后,心衰组外周血IL-10水平(21.93±3.03pg/m1)仍低于普通组(30.50±2.77pg/m1)(p0.05)。结论：呼吸道合胞病毒毛细支气管炎患儿急性期TGF-β和IL-10含量均明显降低,合并心力衰竭患儿尤为显著。恢复期TGF-β和IL-10含量较急性期均明显升高,但浓度仍低于正常儿童。说明TGF-β、IL-10在毛细支气管炎的发病过程中起重要作用。外周血TGF-β、IL-10水平可作为毛细支气管炎病情轻重的指标。
[Abstract]:Objective: Bronchiolitis is the most common pulmonary interstitial inflammation in infants. Respiratory syncytial virus (RSV) is the most common pathogen of bronchiolitis, accounting for more than 70%. Inflammatory cytokines play an important role in the pathogenesis of bronchiolitis. The changes of TGF- 尾 and IL-10 in peripheral blood of children with respiratory syncytial virus capillary bronchitis were detected and its clinical significance was discussed. Subjects and methods of study: From October 2011 to April 2012, 80 children with respiratory syncytial virus capillary bronchitis (throat swab respiratory syncytial virus -PCR positive) hospitalized in Liaocheng people's Hospital were selected as study subjects, according to whether they were complicated with heart failure. They were divided into bronchiolitis group (normal group) and bronchiolitis combined with heart failure group (40 healthy children) as control group. The levels of TGF- 尾 and IL-10 in peripheral blood of children in each group were detected by ELISA double antibody sandwich method. Results: 1. Before treatment, the level of TGF- 尾 in peripheral blood of normal group and heart failure group was 21.77 卤4.47pg / ml and 14.86 卤3.72pg / ml respectively, which was significantly lower than that of control group (53.78 卤4.10pg/ml)(p). 2. Before treatment, the level of TGF- 尾 in peripheral blood of CHF group was 14.86 卤3.72 PG / ml significantly lower than that of normal group (21.77 卤4.47 PG / ml). 3. Before treatment, the IL-10 content in peripheral blood of normal group was 17.55 卤2.47 PG / ml, and that of peripheral blood IL-10 in heart failure group was 10.80 卤2.72 PG / ml, which was significantly lower than that in control group (47.52 卤4.10pg/ml)(p). 4. Before treatment, the level of IL-10 in peripheral blood of CHF group was significantly lower than that of normal group (10.80 卤2.72pg / ml). 5. After treatment, the levels of TGF- 尾 in peripheral blood of the normal group were significantly higher than those of the control group (46.21 卤5.77pg / ml) and 21.77 卤4.47pg / ml, respectively. The level of TGF- 尾 in peripheral blood of heart failure group was 35.68 卤4.03pg / m ~ (-1) significantly higher than that before treatment (14.86 卤3.72 PG / m ~ (-1)). 6. After treatment, the level of TGF- 尾 in peripheral blood of CHF group was 35.68 卤4.03pg / ml, which was still lower than that of normal group (46.21 卤5.77pg/m1)(p, 0.05g / ml). 7. After treatment, the level of IL-10 in peripheral blood of normal group (30.50 卤2.77pg / ml) was significantly higher than that of pre-treatment (17.55 卤2.47pg / ml). The IL-10 content in peripheral blood of heart failure group was 21.93 卤3.03pgP 路m1), which was significantly higher than that before treatment (10.80 卤2.72pg / ml). 8. After treatment, the level of IL-10 in peripheral blood of CHF group (21.93 卤3.03pg / ml) was still lower than that of normal group (30.50 卤2.77pg / ml). Conclusion: The levels of TGF- 尾 and IL-10 in children with respiratory syncytial virus capillary bronchitis decreased significantly in acute phase, especially in children with heart failure. The levels of TGF- 尾 and IL-10 in convalescent stage were significantly higher than those in acute stage, but the concentration of TGF- 尾 and IL-10 was still lower than that of normal children. The results suggest that TGF- 尾 -IL-10 plays an important role in the pathogenesis of bronchiolitis. The level of TGF- 尾 -IL-10 in peripheral blood can be used as an indicator of the severity of bronchiolitis.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.6

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