儿茶酚胺在束缚应激大鼠肺损伤中的作用

发布时间：2018-04-25 14:52

本文选题：肺损伤 + 儿茶酚胺　；参考：《河北医科大学学报》2017年05期

【摘要】：目的研究儿茶酚胺(catecholamine,CA)在束缚应激大鼠肺损伤中的作用。方法选取成年SD雄性大鼠15只,随机分为对照组、束缚应激组、束缚+酪氨酸羟化酶抑制剂(alpha-methyl-p-tyrosine,AMPT)组,每组5只。采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测血浆中去甲肾上腺素(norepinephrine,NE)、肾上腺素(epinephrine,E)水平,苏木素-伊红(hematoxylin-eosin,HE)染色法观察肺组织病理形态学变化,ELISA法检测肺组织中白细胞介素1β(interleukin-1beta,IL-1β)及白细胞介素6(interleukin-6,IL-6)的浓度,二辛可酸法检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中蛋白的含量。结果束缚应激组、束缚+AMPT组血浆中NE、E水平显著高于对照组,束缚+AMPT组血浆中NE、E水平显著低于束缚应激组(P0.05);束缚应激组、束缚+AMPT组肺组织中IL-1β、IL-6浓度和BALF中蛋白含量均显著高于对照组,束缚+AMPT组肺组织IL-1β、IL-6浓度和BALF中蛋白含量显著低于束缚应激组,差异有统计学意义(P0.05)。结论束缚应激可致肺损伤,CA可能参与这一损伤过程,AMPT可明显减轻肺损伤。
[Abstract]:Objective to study the role of catecholamine catecholamine CAA in the lung injury of rats under restraint stress. Methods Fifteen adult Sprague-Dawley rats were randomly divided into control group, restraint stress group and alpha-methyl-p-tyrosine hydroxylase inhibitor AMPT group (5 rats in each group). Enzyme-linked immunosorbent assaysa was used to detect the plasma levels of norepinephrineine (NE) and epinephrineine (E) in plasma by enzyme-linked immunosorbent assay (Elisa). The histopathologic changes of lung tissue were observed by hematoxylin-eosin (HEH) staining. The concentrations of interleukin-1 尾 interleukin-1betasil-1 尾 and interleukin-6 interleukin-6 (IL-6) in lung tissue were detected by Elisa, and the protein content of bronchoalveolar lavage fluidine in bronchoalveolar lavage fluid was detected by dioctanoic acid method. Results in the restraint stress group, the plasma NEI E level in the bound AMPT group was significantly higher than that in the control group, and the plasma NEI E level in the bound AMPT group was significantly lower than that in the restraint stress group (P 0.05). The concentration of IL-1 尾 -IL-6 and the content of BALF protein in lung tissue of bound AMPT group were significantly higher than those of control group, while the concentration of IL-1 尾 -IL-6 and the protein content of BALF in bound AMPT group were significantly lower than those in restraint stress group (P 0.05). Conclusion restraint stress can induce lung injury and CA may be involved in the process of injury. AMPT can significantly reduce lung injury.
【作者单位】：华北理工大学附属医院呼吸内科;河北医科大学法医系河北省法医学重点实验室;河北省胸科医院骨科;河北医科大学第二医院感染科;
【基金】：国家自然科学基金资助课题(81202382)
【分类号】：R563

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