HSP70、NBNA评分与新生儿窒息的相关性研究

发布时间：2018-04-27 13:17

本文选题：HSP70热休克蛋白质类 + 新生儿窒息　；参考：《石河子大学》2017年硕士论文

【摘要】：目的:探讨热休克蛋白70(HSP70)对新生儿窒息的诊断价值及其与不同窒息程度新生儿行为神经测定(NBNA评分)的关系。方法:选择2014年1月至2016年5月本院产科出生的足月窒息新生儿,根据其窒息程度分为轻度窒息组和重度窒息组;另随机选取Apgar评分正常的足月新生儿为对照组。所有入选新生儿生后立即抽取脐动脉血,采用酶联免疫吸附(ELISA)法检测各组HSP70表达水平,并在生后第7、14、28天行NBNA评分,比较各组间HSP70表达水平和NBNA评分与窒息的相关性。结果:共纳入窒息新生儿81例,其中轻度窒息组46例,重度窒息组35例,对照组共纳入50例。各组新生儿脐血HSP70的表达水平差异有统计学意义(P0.05),其中轻、重度窒息组HSP70的表达水平高于对照组[(14.4±2.7)ng/ml、(17.7±2.6)ng/ml比(11.9±2.3)ng/ml,P0.05],且重度窒息组高于轻度窒息组,差异有统计学意义(P0.05)。各组新生儿于生后第7、14、28天行NBNA评分比较差异有统计学意义(P0.05)。相关性分析显示:HSP70水平与NBNA评分(第7、14、28天)呈负相关(r=㧟0.574、㧟0.493、㧟0.208,P均0.05)。新生儿窒息的曲线下面积(AUC)值为0.798(95%CI:0.722~0.874,P0.05),HSP70为12.9 ng/ml,约登指数最大,灵敏度84%,特异度68%。结论:脐血HSP70的表达水平与新生儿窒息程度和NBNA评分相关,窒息程度越重,HSP70表达水平越高,NBNA评分越低。
[Abstract]:Objective: to investigate the diagnostic value of heat shock protein 70 (HSP70) in neonatal asphyxia and its relationship with neonatal behavioral nerve test (NBNA score). Methods: term asphyxia neonates were selected from January 2014 to May 2016 in our hospital and divided into mild asphyxia group and severe asphyxia group according to the degree of asphyxia and the term neonates with normal Apgar score were randomly selected as control group. Umbilical artery blood was collected from all the newborns immediately after birth. The expression of HSP70 in each group was detected by enzyme linked immunosorbent assay (Elisa). The NBNA score was evaluated on the 7th day 1428 after birth, and the correlation between HSP70 expression and NBNA score and asphyxia was compared. Results: 81 cases of asphyxia neonates were included, including 46 cases in mild asphyxia group, 35 cases in severe asphyxia group and 50 cases in control group. The expression of HSP70 in umbilical cord blood of all groups was significantly higher than that of control group (P 0.05). The expression of HSP70 in mild and severe asphyxia group was higher than that in control group [17.7 卤2.6)ng/ml vs 11.9 卤2.3 ng / ml P 0.05], and the difference was statistically significant in severe asphyxia group compared with mild asphyxia group (P 0.05). There was a significant difference in NBNA score between the groups on the 7th day and the 28th day after birth (P 0.05). The correlation analysis showed that there was a negative correlation between the level of NBNA 70 and the NBNA score (7 ~ 14 ~ 28 days). The area under the curve of asphyxia neonatorum was 0.798% 95% CI: 0.72222 0.874P0.05% HSP70 was 12.9 ng / ml, Yorden index was the largest, sensitivity was 84 and specificity was 68. Conclusion: the expression of HSP70 in umbilical cord blood is correlated with the degree of neonatal asphyxia and the NBNA score, and the higher the degree of asphyxia is, the higher the expression level of HSP70 is.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R722.12

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