高脂饲料诱导肥胖大鼠网膜素水平及外源性网膜素的干预作用

发布时间：2018-04-28 10:02

本文选题：网膜素 + 肥胖大鼠　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的：利用高脂饲料喂养大鼠，建立肥胖大鼠模型，比较大鼠在高脂饲料喂养下的体重变化情况、血生化特点、血清网膜素水平及网膜脂肪组织网膜素mRNA表达水平；观察注射外源性网膜素后，肥胖大鼠上述指标的变化，同时探讨外源性网膜素是否对肥胖大鼠具有干预作用。材料与方法：40只SD健康雄性大鼠随机分组：普通饲料组（正常组，n=10），另外30只采用高脂饲料喂养，每周检测体重，高脂饲料喂养6周后选取体重增长值最大的20只，随机分为两组，对照组（n=10）和干预组（n=10）。干预组给予尾静脉注射外源性网膜素0.1ml（6μg），正常组及对照组尾静脉相同体积的注射生理盐水。正常组继续普通饲料喂养，对照组和干预组继续高脂饲料喂养，喂养至10周后再次按上述方案注射，干预组尾静脉注射外源性网膜素剂量为3μg/只，继续喂养至12周。实验结束时三组各剩9只，检测体重后从心脏取血测定空腹血糖（Glucose，GLU）、血清总胆固醇（Total cholesterol，TC）、甘油三酯（Triglyceride，TG）、高密度脂蛋白（High-density lipoprotein，HDL）、低密度脂蛋白（Low-density lipoprotein，，LDL）、空腹胰岛素（Insulin，INS）、血清网膜素。同时取网膜脂肪组织检测网膜素mRNA表达水平。取颈总动脉做病理切片检测动脉内膜厚度。结果：（1）喂养12周后，高脂饲料喂养大鼠（对照组）的体重、体重增加值及肥胖指数（Obesity index，OI）均高于正常饲料喂养组（分别为591.71vs525.98g,286.59vs211.89g,0.049vs0.027, P均0.05）；（2）对照组和干预组的LDL显著高于正常组（0.29vs0.20mmol/L,0.30vs0.20mmol/L, P0.05）；（3）对照组血清网膜素水平及网膜脂肪组织网膜素mRNA表达水平均高于正常组（99.68vs56.98μg/L,1.25vs1.06,P均0.05），干预组与对照组间无差异。（4）干预组肥胖相关指标及动脉内膜厚度（Intima-media thickness，IMT）与对照组相比无显著性差异。结论：首次发现肥胖大鼠其血清网膜素水平及网膜脂肪组织中网膜素mRNA表达水平较正常大鼠显著增高，与人群调查结果不一致。外源性网膜素对肥胖大鼠无干预作用。
[Abstract]:Objective: to establish a model of obese rats fed with high fat diet, and to compare the changes of body weight, blood biochemical characteristics, serum omental hormone level and omentin mRNA expression in omentum adipose tissue of rats fed with high fat diet. Objective: to observe the changes of the above indexes in obese rats after injection of exogenous omentin, and to explore whether exogenous retinin has an intervention effect on obese rats. Materials and methods Forty Sprague-Dawley healthy male rats were randomly divided into two groups: normal diet group (normal group, n = 10), and 30 rats fed with high fat diet, and their body weight was measured weekly. After 6 weeks of high fat diet feeding, 20 rats with the highest weight gain were selected. They were randomly divided into two groups: control group (n = 10) and intervention group (n = 10). The intervention group was injected with exogenous omentin 0.1ml(6 渭 g, and the normal group and the control group were injected with normal saline in the same volume. The normal group was fed with normal diet, the control group and the intervention group continued to be fed with high fat diet. After 10 weeks of feeding, the control group and the intervention group were injected again according to the above plan. The dosage of exogenous omentin in the intervention group was 3 渭 g / mouse by tail vein, and continued to be fed for 12 weeks. At the end of the experiment, the blood samples were collected from the heart to determine fasting blood glucose, total cholesterol (TC), triglyceride-triglyceride (TGG), high density lipoprotein (HDL), low density lipoprotein (LDL), fasting insulin (insulin), and serum omentin. At the same time, omental adipose tissue was taken to detect the expression of omentin mRNA. Carotid artery was taken as pathological section to measure intimal thickness. Results after 12 weeks of feeding, the weight of rats fed with high-fat diet (control group), The LDL of the control group and the intervention group were significantly higher than that of the normal diet feeding group (591.71 vs 525.98 g / 286.59 vs 211.89 vs 0.049 vs 0.027, P = 0.05 respectively). The LDL of the control group and the intervention group were significantly higher than that of the normal group (0.29 vs 0.20 mmol / L 0.30 vs 0.20 mmol / L, P 0.05%). The omentum hormone level and omentum adipose tissue omentofiline mRNA table in the control group were significantly higher than those in the normal diet group (591.71 vs 525.98 g / L 286.59 vs 211.89 vs 0.049 vs 0.027, P < 0.05 respectively). There was no significant difference in obesity related indexes and intimal thickness between intervention group and control group (P > 0.05). There was no significant difference between intervention group and control group. Conclusion: the level of omentin in serum and the expression of omentin mRNA in omental adipose tissue of obese rats were significantly higher than that of normal rats for the first time, which was not consistent with the results of population investigation. There was no intervention effect of exogenous omentin on obese rats.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R723

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