重组融合蛋白IL-18对金黄色葡萄球菌感染小鼠免疫相关炎症因子表达的影响

发布时间：2018-05-01 16:04

  本文选题：IL- + 金黄色葡萄球菌　； 参考：《中国当代儿科杂志》2017年06期

【摘要】：目的观察重组融合蛋白IL-18对金黄色葡萄球菌(SA)感染后小鼠体内免疫相关炎症因子表达的影响,探讨IL-18在体内防御SA感染的可能机制。方法将40只SPF级雌性BLAB/c小鼠随机分为对照组、SA感染组、免疫组和干预组。采用鼻腔接种SA液建立SA感染小鼠模型,免疫组和干预组均在建模前以IL-18滴鼻,但免疫组不予SA接种,对照组以PBS进行替代处理。采用ELISA法测定各组小鼠血液及支气管肺泡灌洗液(BALF)中IL-4、干扰素-γ(IFN-γ)、肿瘤坏死因子(TNF)、粒细胞集落刺激因子(G-CSF)、Ig M的浓度。实时荧光定量PCR技术检测各组小鼠肺组织中巨噬细胞炎性蛋白(MIP)-1α和MIP-2βm RNA表达水平。结果与对照组相比,SA感染组和免疫组小鼠血清及BALF中IL-4、G-CSF、Ig M浓度,以及肺组织中MIP-1α、MIP-2βm RNA含量均升高(P0.05);SA感染组小鼠血清及BALF中IFN-γ水平降低,TNF水平升高(P0.05);免疫组小鼠血清及BALF中IFN-γ水平升高(P0.05)。与SA感染组相比,干预组小鼠血清及BALF中IL-4、IFN-γ、G-CSF、Ig M浓度,以及肺组织中MIP-1αm RNA含量均升高,血清及BALF中TNF水平,以及肺组织中MIP-2βm RNA含量均降低(P0.05)。除血清IFN-γ水平外,其余上述指标在干预组小鼠中均高于对照组(P0.05)。结论重组融合蛋白IL-18经黏膜免疫小鼠,可改变SA感染后小鼠血清及BALF中炎症因子,以及肺组织中MIP-1α、MIP-2βm RNA的表达水平,从而促进机体的抗感染免疫反应,增强了机体清除病原体的能力。
[Abstract]:Objective to observe the effect of recombinant fusion protein IL-18 on the expression of immune-associated inflammatory factors in mice infected with staphylococcus aureus and to explore the possible mechanism of IL-18 in the defense of SA infection in vivo. Methods 40 SPF female BLAB/c mice were randomly divided into three groups: control group, immune group and intervention group. The model of SA-infected mice was established by nasal inoculation with SA solution. Both the immune group and the intervention group were given IL-18 nasal drip before modeling, but the immune group was not inoculated with SA, and the control group was treated with PBS. The concentrations of IL-4, IFN- 纬, TNFF and G-CSF- IFN- 纬 in blood and bronchoalveolar lavage fluid (BALF) of mice in each group were determined by ELISA method. The expression of macrophage inflammatory protein (MIP-1 伪) and MIP-2 尾 m RNA in lung tissue of mice in each group was detected by real-time fluorescence quantitative PCR. Results compared with the control group, the serum and BALF levels of IL-4G-CSFG in the SA infected and immunized mice were higher than those in the control group. The levels of IFN- 纬 in serum and BALF of mice infected with P0.05 were increased, and the levels of IFN- 纬 in serum and BALF of immunized mice were increased. Compared with SA infection group, the concentration of IL-4 IFN- 纬 G-CSFFG G M in serum and BALF, the content of MIP-1 伪 m RNA in lung tissue, the TNF level in serum and BALF, and the content of MIP-2 尾 m RNA in lung tissue in the intervention group were all lower than those in the control group (P 0.05). With the exception of serum IFN- 纬, the above indexes in the intervention group were higher than those in the control group (P 0.05). Conclusion the recombinant fusion protein IL-18 can change the levels of inflammatory factors in serum and BALF and the expression level of MIP-1 伪 -MIP-2 尾 m RNA in lung tissue of SA-infected mice, thus promoting the anti-infection immune response. Enhanced the body's ability to remove pathogens.
【作者单位】： 江西省儿童医院呼吸内科;
【分类号】：R725.1
，

本文编号：1830081