γ-促分泌酶抑制剂阻断Notch信号通路对毛细支气管炎模型大鼠气道炎症的影响

发布时间：2018-05-01 22:41

本文选题：γ-促分泌酶抑制剂 + 毛细支气管炎　；参考：《现代免疫学》2017年04期

【摘要】：为研究γ-促分泌酶抑制剂阻断Notch信号对毛细支气管炎(简称毛支)模型大鼠气道炎症反应的影响,建立毛支大鼠模型并且尾静脉注射γ-促分泌酶抑制剂(MW167)。采用HE染色法观察肺组织病理改变,ELISA方法检测血浆中IL-4、INF-γ的水平,real-time PCR检测肺组织和外周血单个核细胞中IL-4mRNA、IFN-γmRNA的表达,Western blotting检测肺组织中NICD、GATA-3、T-bet的蛋白表达。结果显示,MW167注射组较RSV毛支组病理学改变有所减轻;MW167注射组血浆中IL-4水平较RSV组降低,IFN-γ水平增高;肺组织及外周血单个核细胞IL-4mRNA在MW167注射组表达减弱,IFN-γmRNA表达增强;Notch信号和GATA-3表达降低,T-bet表达增强。以上结果提示静脉注射γ-促分泌酶抑制剂能够抑制毛支模型大鼠的气道炎症,可能对毛支有潜在的治疗价值。
[Abstract]:In order to study the effect of 纬 -secretory enzyme inhibitor blocking Notch signal on airway inflammation in rat model of bronchiolitis (hair branch), a rat model of bronchiolitis was established and tail vein injection of 纬 -secretory enzyme inhibitor (MW167) was performed. The expression of IL-4mRNA-IFN- 纬 mRNA in lung tissues and peripheral blood mononuclear cells (PBMC) was detected by real-time PCR. The expression of NICDD-GATA-3T-bet in lung tissues was detected by Western blotting. The results showed that the histopathological changes of MMW 167 injection group were decreased compared with that of the RSV hair branch group. The plasma IL-4 level in the MMW 167 injection group was lower than that in the RSV group, and the level of IFN- 纬 was increased. The expression of IL-4mRNA in lung tissue and peripheral blood mononuclear cells decreased in MW167 injection group. The expression of IFN- 纬 mRNA increased. The signal of Notch and the expression of GATA-3 decreased the expression of T-bet. These results suggest that intravenous injection of 纬 -secretory stimulating enzyme inhibitor can inhibit airway inflammation in the rat model of hair branch, which may have potential therapeutic value to the hair branch.
【作者单位】：滨州医学院;滨州医学院附属医院;
【基金】：山东省自然科学基金(ZR2014HL001)
【分类号】：R-332;R725.6

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