抵抗素、miRNA-26b在婴幼儿毛细支气管炎中的表达

发布时间：2018-05-02 18:18

本文选题：抵抗素 + microRNA　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的探讨婴幼儿毛细支气管炎中miRNA-26b、抵抗素的变化。方法收集毛细支气管炎患儿的血液,以正常儿童为对照,通过酶联免疫吸附(ELISA)法测定血清中白介素(IL)-8、抵抗素浓度,并利用实时定量PCR技术测定血液淋巴细胞中miRNA-26b、抵抗素mRNA表达,利用miRNA-26b mimics干预人肺上皮细胞A549,通过实时定量PCR技术测定miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度变化。结果毛细支气管炎患儿外周血清中IL-8、抵抗素浓度均升高,血液淋巴细胞中miRNA-26b、抵抗素mRNA表达增加;经过干预的A549细胞miRNA-26b、抵抗素mRNA及细胞上清液中IL-8蛋白浓度均有增加。结论 miRNA-26b、抵抗素在毛细支气管炎患儿血液淋巴细胞中存在差异表达,而这可能参与了炎症反应的发生。
[Abstract]:Objective to investigate the changes of miRNA-26 b and resistin in infants with bronchiolitis. Methods Blood samples from children with bronchiolitis were collected and compared with normal children. The concentrations of interleukin-8 and resistin in serum were determined by Elisa, and the expression of miRNA-26b and resistin mRNA in blood lymphocytes was measured by real-time quantitative PCR technique. Human lung epithelial cells A549 were treated with miRNA-26b mimics. The concentration of miRNA-26b, resistin mRNA and IL-8 protein in supernatant of human lung epithelial cells were measured by real-time quantitative PCR. Results the concentrations of IL-8 and resistin in peripheral serum of children with bronchiolitis were all increased, miRNA-26b and resistin mRNA expression in blood lymphocytes were increased, and miRNA-26b, resistin mRNA and IL-8 protein in supernatant of A549 cells were increased after intervention. Conclusion miRNA-26b, resistin is differentially expressed in blood lymphocytes of children with bronchiolitis, which may be involved in inflammatory reaction.
【作者单位】：南京医科大学附属儿童医院呼吸科;
【基金】：国家自然科学基金(编号:81200012)
【分类号】：R725.6

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