一起疑似病毒性脑膜炎暴发病原学检测分析

发布时间：2018-05-09 05:37

本文选题：暴发 + 病毒性脑膜炎　；参考：《中国公共卫生》2017年02期

【摘要】：目的 2014年10月浙江省金华市荣光小学发生疑似病毒性脑膜炎暴发疫情,为及时查明病因,分析病原的分子特征并进行病原溯源。方法开展流行病学调查,采集患者脑脊液和咽拭子样本6份,检测人类肠道病毒(HEV)核酸和分离病毒,对HEV分离株VP1基因测序,进行同源性与进化分析。结果在全校328名学生中,发病7例,罹患率2.13%。患者年龄7~8岁;临床症状为发热、头痛、呕吐等;从5例患者6份样本中均检测到HEV核酸,其中3份脑脊液中分离到3株肠道病毒埃可30型(echovirus 30,E30);新分离株与E30原型株之间VP1区核苷酸和氨基酸同源性分别为81.8%和91.8%~92.1%;进化分析显示:新分离株位于E30 G基因亚型分支上,与2013年浙江省CHN/ZJ/RA-72/13株亲缘关系最近。结论引起2014年10月浙江省金华市荣光小学聚集性病毒性脑膜炎暴发疫情的病原为E30 G基因亚型病毒。
[Abstract]:Objective in October 2014, a suspected viral meningitis outbreak occurred in Rongguang Primary School of Jinhua City, Zhejiang Province. Methods six samples of cerebrospinal fluid (CSF) and throat swabs were collected from patients. The nucleic acid of human enterovirus (HEV) and the isolated virus were detected. The VP1 gene of HEV isolate was sequenced, and the homology and evolution were analyzed. Results among 328 students, there were 7 cases, the attack rate was 2.13%. The patients were aged 78 years, with symptoms of fever, headache, vomiting, etc. HEV nucleic acid was detected in 6 samples of 5 patients. Three strains of enterovirus E30 echovirus E30 were isolated from cerebrospinal fluid. The nucleotide and amino acid homology of the VP1 region between the new isolate and the E30 prototype strain were 81.8% and 91.8%, respectively. Evolutionary analysis showed that the new isolate was located on the E30G gene subtype branch. It has the closest relationship with the CHN/ZJ/RA-72/13 strain in Zhejiang province in 2013. Conclusion the E30G gene subtype virus was the cause of the outbreak of aggregated viral meningitis in Rongguang Primary School in Jinhua City of Zhejiang Province in October 2014.
【作者单位】：浙江省疾病预防控制中心微生物检验所病毒检验科;
【基金】：浙江省科技厅项目(2012C33063)
【分类号】：R725.1

【参考文献】