维生素D对脓毒症幼鼠肺树突状细胞表面分子CD83、CD86的影响

发布时间：2018-05-09 22:13

本文选题：维生素D + 脓毒症　；参考：《南昌大学》2017年硕士论文

【摘要】：目的:探讨维生素D对脓毒症大鼠肺树突状细胞表面分子CD83、CD86表达水平及抗炎因子IL-10、促炎因子IL-12分泌水平的影响。方法:将雄性(SD)幼年大鼠24只按随机数字表法均分为正常对照组(Ctrl组),脓毒症组(LPS组)和维生素D(VitD)干预组(VitD组)。腹腔注射脂多糖(LPS5mg/kg)[1]制备大鼠脓毒症模型,VitD组在制备脓毒症模型之前48h、24h及1h腹腔内注射VitD3(1000U/kg)[2]。脓毒症模型制备后12小时收集各组大鼠血液及肺组织。用流式细胞仪检测肺DCs表面分子CD83和CD86的表达;采用酶联免疫吸附试验(ELISA)检查肺组织中促炎因子(IL-12)和抗炎因子(IL-10)的含量;采用ELISA法检查各组大鼠血清25(OH)D水平;肺组织HE染色观察其病理改变。结果:幼年大鼠注射LPS后,肺DCs表面分子CD86和CD83的表达增高,与正常对照组相比较表达率明显上升,差异显著(P0.05),但VitD组肺DC表面分子CD86和CD83的表达较LPS组降低,与LPS组比较差异显著,具有统计学意义(P0.05);LPS组肺组织匀浆上清液中IL-12的含量较高,而IL-10的水平较低,以上两种炎症因子与正常对照组相比较均具有统计学差异。VitD组肺组织促炎因子IL-12的分泌水平较LPS组降低,而抗炎因子IL-10水平较LPS组显著升高,且均有统计学差异(P0.05);三组中,LPS组血清25(OH)D水平最低,VitD组血清25(OH)D水平较LPS组升高,具有显著差异(P均0.05),但两组均明显低于正常对照组;肺组织病理检测显示LPS组肺组织病理改变显著,而VitD组肺组织病理改变轻于LPS组。结论:1、脓毒症动物模型制备成功。2、脓毒症幼鼠肺组织中DCs表面分子CD83、CD86表达增加,炎症因子中促炎因子IL-12分泌增加,IL-10水平降低,提示DCs的活化、炎症因子的分泌参与了脓毒症的发生、发展。3、VitD可抑制脓毒症幼鼠肺DCs表面分子CD83、CD86的表达,促进抗炎因子IL-10的分泌,抑制促炎因子IL-12的释放,减轻了机体过度的炎症反应。
[Abstract]:Aim: to investigate the effects of vitamin D on the expression of CD83- and CD86 on the surface of pulmonary dendritic cells and the secretion of anti-inflammatory cytokines IL-10 and pro-inflammatory factor IL-12 in septic rats. Methods: Twenty-four young male SD rats were randomly divided into normal control group (Ctrl group), septic group (LPS group) and vitamin D Vitamin D (VitD) intervention group (Vit D group). Lipopolysaccharide (LPS 5 mg / kg) [1] the sepsis model of rats was induced by intraperitoneal injection of VitD (1000Ukg) at 48h, 24h and 1h before the model of sepsis was established. Blood and lung tissues were collected 12 hours after sepsis model. The expression of CD83 and CD86 on lung DCs was detected by flow cytometry, the levels of proinflammatory factor IL-12 (IL-12) and anti-inflammatory factor (IL-10) in lung tissue were detected by Elisa, and the serum 25(OH)D level of rats was detected by ELISA method. The pathological changes of lung tissue were observed by HE staining. Results: after injection of LPS, the expression of CD86 and CD83 on lung DCs surface in young rats was increased, and the expression rate of CD86 and CD83 was significantly higher than that of normal control group (P 0.05). However, the expression of CD86 and CD83 on lung DC in VitD group was lower than that in LPS group. Compared with LPS group, the content of IL-12 in lung homogenate supernatant was higher, but the level of IL-10 was lower. The level of IL-12 in lung tissue in Vit D group was lower than that in LPS group, while the IL-10 level of anti-inflammatory factor in Vit D group was significantly higher than that in LPS group. The level of serum 25(OH)D in 25(OH)D group was significantly higher than that in LPS group (P 0.05), but it was significantly lower in both groups than that in normal control group. The pathological changes of lung tissue in LPS group were significantly higher than that in LPS group, while that in VitD group was less than that in LPS group. Conclusion: 1, the animal model of sepsis was successfully established. The expression of CD83 CD86 on the surface of DCs and the secretion of proinflammatory factor IL-12 in inflammatory factors were increased and the level of IL-10 was decreased in the lung tissue of septic young rats, indicating the activation of DCs. The secretion of inflammatory factors is involved in the occurrence of sepsis. The development of Vit D can inhibit the expression of CD83 CD86 on the surface of pulmonary DCs, promote the secretion of anti inflammatory factor IL-10, inhibit the release of proinflammatory factor IL-12, and alleviate the excessive inflammatory reaction of the body.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R720.597

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