急性期过敏性紫癜患儿Tfh细胞改变及意义初探

发布时间：2018-05-11 02:10

本文选题：滤泡辅助性T细胞 + Bcl-6/Blimp-1　；参考：《重庆医科大学》2012年硕士论文

【摘要】：目的：探讨滤泡辅助性T细胞(Tfh细胞)在儿童过敏性紫癜中的数量变化及其可能机制。方法：过敏性紫癜(HSP)急性期患儿20例,采用流式细胞术检测外周血CD4+CXCR5+ICOS+T(?)田胞(Tfh)的比例,采用荧光定量聚合酶链反应(Real-time PCR)检测转录因子Bcl-6及其拮抗因子Blimp-1的mRNA表达,酶联免疫吸附试验检测IL-21、IL-4血浆浓度。结果：①HSP急性期患儿Tfh细胞比例明显高于正常对照组(2.9±1.0%vs1.8±0.72%,P0.05)。②Tfh细胞正性转录调节因子BCl-6mRNA表达较正常对照组明显增高(P0.05),负性调节因子Blimp-1mRNA表达降低(P0.05)。③Tfh细胞相关细胞因子IL-21血浓度明显高于正常对照组(P0.05),抑制Blimp-1表达的IL-4血浆浓度明显高于正常对照组(P0.05)。结论：Tfh细胞过度活化可能参与HSP免疫发病机制,Bcl-6/Blimp-1表达失衡,IL-21、IL-4细胞因子微环境改变可能与Tfh细胞异常活化有关。
[Abstract]:Objective: to investigate the quantitative changes and possible mechanism of follicular helper T cell (Tfh) in children with allergic purpura. Methods: twenty children with acute stage of Henoch-Schonlein purpura (CD4 CXCR5 ICOS) were detected by flow cytometry. The mRNA expression of transcription factor Bcl-6 and its antagonist Blimp-1 was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR) and the plasma concentration of IL-21 and IL-4 was detected by enzyme-linked immunosorbent assay (Elisa). Results the percentage of Tfh cells in children with HSP1 in acute phase was significantly higher than that in control group (2.9 卤1.0%vs1.8 卤0.72) and the expression of positive transcription regulator BCl-6mRNA was significantly higher than that in normal control group. The expression of negative regulatory factor Blimp-1mRNA decreased P0.05n.3Tfh cell related fine cells. The plasma concentration of cytokine IL-21 was significantly higher than that of normal control group (P 0.05), and the plasma concentration of IL-4 which inhibited the expression of Blimp-1 was significantly higher than that of normal control group (P 0.05). Conclusion the overexpression of Tfh cells may be involved in the pathogenesis of HSP. The microenvironment changes of IL-21 and IL-4 cytokines may be related to the abnormal activation of Tfh cells.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.5

【共引文献】