氯胺酮对新生大鼠学习记忆及海马乙酰胆碱酯酶、乙酰胆碱转移酶的影响

发布时间：2018-05-12 16:24

本文选题：氯胺酮 + 海马　；参考：《安徽医科大学》2012年硕士论文

【摘要】：目的：探讨氯胺酮对新生大鼠学习记忆能力和海马乙酰胆碱酯酶(AchE)、乙酰胆碱转移酶(ChAT)的影响。方法：80只7d龄SD大鼠，，体重12～18g，雌雄不拘，随机分为C、K1、K2、K3组，每组20只。C组单次腹腔注射（i.p.）生理盐水2ml；K1组单次注射氯胺酮80mg/kg i.p.；K2组连续2d给予氯胺酮，80mg/kg.d i.p.，K3组连续3d给予氯胺酮，80mg/kg.d i.p.。给药过程结束后1d各组处死大鼠10只（首批处死）并立即取左、右脑海马组织以比色法分别观察乙酰胆碱转移酶、乙酰胆碱酯酶表达情况；其余各组大鼠饲养至21d龄采用Morris水迷宫系统观察学习记忆能力改变情况，水迷宫行为测试结束后处死大鼠（27d龄处死），立即取左、右脑海马组织比色法分别观察ChAT、AchE表达情况。大鼠的海马组织ChAT、AchE活力和学习记忆能力变化结果采用统计软件SPSS13.0分析比较。结果：⑴Morris水迷宫：C组与K1组之间比较、K2组与K3组之间比较差异均无显著性(P0.05)；K2、K3组较C、K1组潜伏期延长且穿越平台次数减少，差异有显著性(P0.05)。⑵酶测定：首批处死后，C、K1、K2、K3各组间差异有显著性(P0.05)。27d龄处死，C组与K1组之间比较、K2组与K3组之间比较差异均无显著性(P0.05)；K2、K3组较C、K1组差异均有显著性(P0.05)。结论：氯胺酮80mg/kg可引起7日龄SD大鼠海马胆碱能系统ChAT、AchE活力改变。单次给药可引起大鼠24h内海马胆碱能系统ChAT、AchE活力变化；重复给药可引起24h内及21天后海马胆碱能系统ChAT、AchE活力变化；随着给药次数增加所引起的24h内海马胆碱能系统ChAT、AchE活力变化也不相同。单次给药未见引起大鼠21天后空间学习记忆能力改变；重复给药能引起21天后空间学习记忆能力改变；而重复给药后再次给药并未见明显ChAT、AchE与空间学习记忆能力改变。SD大鼠海马胆碱能系统ChAT、AchE参与了空间学习记忆功能，氯胺酮引起空间学习记忆能力的改变可能与其影响海马胆碱能神经乙酰胆碱酯酶、乙酰胆碱转移酶有关。
[Abstract]:Aim: to investigate the effects of ketamine on learning and memory ability and hippocampal acetylcholinesterase (ache) in neonatal rats. Methods Twenty seven day old SD rats, weighing 12g or 18g, were randomly divided into two groups: group C (n = 20) and group C (n = 20). Normal saline 2ml / L K1 group was given Ketamine 80 mg / kg / kg i.p. K3 for 3 consecutive days by single injection of ketamine 80mg/kg i.p. K2 for 2 consecutive days, and ketamine 80 mg / kg 路d i.p. Ten rats in each group were killed 1 day after the administration (the first group was executed) and the expression of acetylcholine transferase and acetylcholinesterase were observed by colorimetric method in the hippocampus of left and right brain. Morris water maze system was used to observe the changes of learning and memory ability in the other groups. After the water maze behavior test, the rats were killed at the age of 27 days, and the expression of Chattoache was observed by colorimetric method in the hippocampus of left and right brain. The changes of ache activity and learning and memory ability in hippocampus of rats were analyzed and compared by SPSS13.0 software. Results there was no significant difference between K2 group and K3 group compared with K1 group. The latency of K2 group was longer and the number of crossing platform was lower than that of Con K1 group, and there was no significant difference between K3 group and K1 group, and there was no significant difference between K2 group and K1 group, compared with K1 group, there was no significant difference between K2 group and K3 group. There was a significant difference in P0.05.2 enzyme: after the first group was executed, there was significant difference among the three groups. There was no significant difference between K2 group and K3 group compared with K1 group. There was no significant difference in P0.05K2K3 group and CnK1 group in comparison with that in C0. 05K1 group. There was no significant difference between K2 group and K3 group. Conclusion: ketamine 80mg/kg can induce the changes of cholinergic system activity of cholinergic system in 7 day old SD rats. The changes of Chatache activity in hippocampal cholinergic system were induced by single administration of the drug within 24 hours, and the activity of Chatache in hippocampal cholinergic system by repeated administration within 24 hours and 21 days after administration. The changes of ChATache activity in hippocampal cholinergic system were different with the increase of administration times in 24 hours. No change of spatial learning and memory ability was found in rats after 21 days of single administration, but repeated administration of drugs caused changes in spatial learning and memory ability after 21 days. However, after repeated administration, there was no obvious difference between ChATAache and spatial learning and memory ability. The cholinergic system ChATAache in hippocampus of SD rats was involved in spatial learning and memory function. The change of spatial learning and memory induced by ketamine may be related to the effect of ketamine on cholinergic acetylcholinesterase and acetylcholine transferase in hippocampal cholinergic nerve.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R965;R722.1

【参考文献】