新生期间断重复吸入七氟醚对大鼠学习记忆及Tau蛋白和p-Tau蛋白的影响

发布时间：2018-05-21 19:14

本文选题：七氟醚 + 海马　；参考：《医学研究生学报》2017年03期

【摘要】：目的学习记忆功能是大脑的高级神经活动形式,一旦大脑海马组织受损,可严重影响患者的生活质量。文中旨在观察新生期大鼠间断重复吸入七氟醚对其幼年期、成年期学习记忆功能以及大脑海马中Tau蛋白、p-Tau蛋白的影响。方法 24只健康新生SD大鼠,采用随机数字表法分为幼年期七氟醚吸入组、幼年期对照组、成年期七氟醚吸入组和成年期对照组,每组6只。幼年期七氟醚吸入组、成年期七氟醚吸入组分别于出生后第7、14、21天(记录P7、P14、P21)吸入2.6%七氟醚2h,幼年期对照组、成年期对照组则在相同的时间点吸入运载气体(1L/min O2+1 L/min Air)2 h。幼年期七氟醚吸入组与幼年期对照组于P31-37,成年期七氟醚吸入组与成年期对照组于P91-97行Morris水迷宫实验检测其行为学,然后取其海马组织,检测海马中Tau蛋白与p-Tau蛋白表达水平。结果幼年期和成年期七氟醚吸入组与其对照组逃避潜伏期同时间点比较差异无统计学意义(P0.05)。空间探索实验结果比较:幼年期与成年期大鼠在原平台区域进入次数、游泳滞留时间、游泳路程以及游泳速度等组间比较差异均无统计学意义(P0.05)。Tau蛋白表达检测:与幼年期对照组海马CA1、CA3、DG区表达(0.185±0.024、0.232±0.040、0.184±0.018)相比,幼年期七氟醚吸入组(0.237±0.015、0.324±0.024、0.226±0.019)明显增高(P0.01);成年期七氟醚吸入组与其对照组比较差异无统计学意义(P0.05)。p-Tau蛋白表达检测:与成年期对照组海马CA3区表达(0.158±0.011)相比,成年期七氟醚吸入组表达水平(0.170±0.005)增高(P0.05);而幼年期七氟醚吸入组与其对照组比较差异无统计学意义(P0.05)。结论新生期间断重复吸入七氟醚麻醉未见损害大鼠在幼年期和成年期的学习记忆功能,推测其可能与幼年期增加的Tau蛋白和成年期增加的p-Tau蛋白表达并不足以引起其学习记忆功能下降有关。
[Abstract]:Objective Learning and memory is a form of advanced neural activity in the brain. Once the hippocampal tissue is damaged, it can seriously affect the quality of life of patients. The purpose of this study was to observe the effects of intermittent repeated inhalation of sevoflurane on learning and memory function and Tau protein p-Tau protein in hippocampus of neonatal rats during infancy and adulthood. Methods Twenty-four healthy newborn SD rats were randomly divided into three groups: the infant sevoflurane inhalation group, the juvenile control group, the adult sevoflurane inhalation group and the adult control group with 6 rats in each group. In the infant sevoflurane inhalation group, the adult sevoflurane inhalation group inhaled 2.6% sevoflurane for 2 h on the 7th day after birth (recorded P7 P14P14P21), while the juvenile control group and the adult control group inhaled transport gas 1 L / min O 2 1 L/min Air)2 h at the same time point. The behavior of sevoflurane inhalation group was detected by Morris water maze test, and the expression of Tau protein and p-Tau protein was detected by Morris water maze test in adult sevoflurane inhalation group and juvenile control group. Results there was no significant difference in escape latency between infant and adult sevoflurane inhalation group and control group at the same time point (P 0.05). Comparison of the results of space exploration experiments: the entry times and swimming retention time of juvenile and adult rats in the original platform area were compared. There was no significant difference in swimming distance and swimming speed between the two groups. There was no significant difference in the expression of P0. 05. Tau protein between the two groups: compared with the young control group, the expression of P0. 05 卤0. 024, 0. 232 卤0. 040 and 0. 184 卤0. 018 in the hippocampal CA1 + CA3 + DG region of the juvenile control group. In juvenile sevoflurane inhalation group, 0.237 卤0.015 卤0.324 卤0.024 卤0.226 卤0.019) were significantly increased, and there was no significant difference between adult sevoflurane inhalation group and control group in the expression of P0.05, p-Tau protein: compared with adult control group, the expression of CA3 in hippocampus was 0.158 卤0.011), and that in adult sevoflurane inhalation group was 0. 158 卤0. 011), and there was no significant difference between adult sevoflurane inhalation group and control group. In adult sevoflurane inhalation group, the expression level was 0.170 卤0.005), but there was no significant difference between infant sevoflurane inhalation group and control group. Conclusion repeated inhalation of sevoflurane during neonate did not damage the learning and memory function of rats in infancy and adulthood. It is suggested that the increased expression of Tau protein in infancy and the increased expression of p-Tau protein in adulthood may not be sufficient to induce the decline of learning and memory function.
【作者单位】：贵州省麻醉与器官保护基础研究重点实验室;
【基金】：国家自然科学基金(81360180)
【分类号】：R722.1

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