咳喘宁口服液对哮喘模型大鼠中核因子-κB p65和TLR4的影响

发布时间：2018-05-25 01:02

本文选题：咳喘宁口服液 + 哮喘大鼠　；参考：《湖南中医药大学》2014年硕士论文

【摘要】：目的：通过建立SD(Sprague-Dawley)大鼠哮喘模型,采用免疫组化的方法,检测哮喘大鼠肺组织中核因子-κB p65及TLR4的表达水平,探讨核因子-κB p65及TLR4在哮喘发病中的表达变化以及咳喘宁对其的影响。方法：将50只SPF级雄性SD大鼠随机分为：正常组,不做任何处理；模型组,用卵清蛋白诱发哮喘；咳喘宁组,使用卵清蛋白诱发哮喘后,用中药咳喘宁口服液治疗；布地奈德组,用卵清蛋白诱喘后用布地奈德治疗。采用免疫组化法检测各组肺组织中核因子-κB p65及TLR4的水平；采用全自动血细胞计数仪对肺泡灌洗液中白细胞总数、中性粒细胞、嗜酸性粒细胞(EOS)及淋巴细胞计数；并观察支气管肺组织的病理形态学改变。结果： 1.各组大鼠支气管肺泡洗灌液的细胞计数及分类计数：模型组BALF中白细胞总数、中性粒细胞数、淋巴细胞数及EOS均升高(P0.05)；咳喘宁组与模型组相较,BALF中白细胞总数、淋巴细胞数、EOS均降低(P0.05)；咳喘宁组与布地奈德组相较,BALF中各细胞数无明显差异(P0.05)。 2.各组大鼠肺组织病理变化：正常组无明显炎症改变。模型组呈显著炎性变化。布地奈德组与咳喘宁组均呈轻度炎症变化。 3.各组大鼠肺组织核因子-κB p65免疫组化表达：核因子-κB p65在正常组大鼠肺组织呈弱阳性表达；模型组大鼠肺组织内核因子-κBp65的表达明显较正常组增加；布地奈德组和咳喘宁组核因子-κB p65的表达强于正常组,弱于模型组,布地奈德组与咳喘宁组比较核因子-κB p65表达无明显差异。 4.各组大鼠肺组织TLR4免疫组化表达：TLR4在正常组大鼠肺组织呈弱阳性表达；模型组大鼠肺组织内TLR4的表达明显较正常组增加；布地奈德组和咳喘宁组TLR4的表达强于正常组,弱于模型组,布地奈德组与咳喘宁组比较TLR4表达无明显差异。结论：咳喘宁口服液能减轻哮喘大鼠肺组织的炎症反应；模型组大鼠肺组织中TLR4、NF-κB P65的阳性表达明显升高,咳喘宁口服液治疗哮喘能降低TLR4、NF-κB P65的表达。
[Abstract]:Objective: to study the expression of nuclear factor- 魏 B p65 and TLR4 in lung tissue of SD rague-Dawley (SD) rats by immunohistochemical method. To investigate the expression of nuclear factor- 魏 B p65 and TLR4 in asthma and the effect of Kechuanning on them. Methods: fifty SPF male SD rats were randomly divided into normal group without any treatment, model group with ovalbumin induced asthma and Kechuanning group with Kechuanning oral liquid. Budesonide group was treated with budesonide after ovalbumin induced asthma. The levels of nuclear factor- 魏 B p65 and TLR4 in lung tissue were detected by immunohistochemical method, and the total number of leukocytes, neutrophils, eosinophils and lymphocytes in alveolar lavage fluid were counted by automatic blood cell counter. The histopathological changes of bronchopulmonary tissue were observed. Results: 1. The cell count and classification count of bronchoalveolar lavage fluid in each group: the total number of leukocytes, neutrophils, lymphocytes and EOS in BALF of model group were all increased (P 0.05), and the total number of leukocytes in Kechuanning group was higher than that in model group. In Kechuanning group and budesonide group, there was no significant difference in the number of cells in BALF between Kechuanning group and budesonide group. 2. Pathological changes of lung tissue in each group: there was no obvious inflammatory change in normal group. The model group showed significant inflammatory changes. Both budesonide group and Kechuanning group showed mild inflammation. 3. The expression of nuclear factor- 魏 B p65 was weakly positive in the normal group, and the expression of nuclear factor- 魏 B p65 in the model group was significantly higher than that in the normal group. The expression of NF- 魏 B p65 in budesonide group and Kechuanning group was higher than that in normal group, but weaker than that in model group. There was no significant difference in the expression of NF- 魏 B p65 between budesonide group and Kechuanning group. 4. The expression of TLR4 was weakly positive in the normal group, the expression of TLR4 in the model group was significantly higher than that in the normal group, and the expression of TLR4 in the budesonide group and Kechuanning group was stronger than that in the normal group. There was no significant difference in TLR4 expression between budesonide group and Kechuanning group. Conclusion: Kechuanning oral liquid can attenuate the inflammatory reaction of lung tissue in asthmatic rats, and the positive expression of TLR4 and NF- 魏 B p65 in lung tissue of model group is obviously increased, while Kechuanning oral liquid can reduce the expression of TLR4- 魏 B p65 in asthmatic rats.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R272;R285.5

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