Semaphorin 5A在先天性巨结肠症中的表达

发布时间：2018-06-07 09:47

本文选题：Semaphorin5A + 先天性巨结肠症　；参考：《遵义医学院》2012年硕士论文

【摘要】：目的：先天性巨结肠症(Hirschsprung disease, HSCR)是一种以肠肌间神经丛神经节细胞缺失为主要特征的疾病,可累计部分或全部肠管。目前已知肠神经元前体细胞(ENPs)的迁移异常为先天性巨结肠症的发病机制之一,而Semaphorin5A在ENPs的迁移中起着重要的作用。本研究试图分析Semaphorin5A蛋白在儿童先天性巨结肠中的表达情况,并初步探讨Semaphorin5A与先天性巨结肠症的关系。方法：选取深圳市儿童医院手术治疗的先天性巨结肠症患儿结肠标本共31例,所有患儿均由手术切除标本送病理活检确诊。将先天性巨结肠症患儿的无神经节细胞的肠组织设为实验组,将有神经节细胞的肠组织设为对照组,实验组的标本均经过病理检查证实无神经节,对照组的标本均经过病理检查证实含有神经节。通过免疫荧光和Western-blot技术检测所有患儿的有神经节细胞的肠组织及无神经节细胞的肠组织中的Semaphorin5A表达情况,并将研究数据通过t检验分析,P03.05为差异具有统计学意义。结果：免疫荧光结果显示,Semaphorin5A可表达于所有标本的肠肌层的神经节细胞、粘膜下层、环形肌和纵行肌中。Western-blot分析结果显示,Semaphorin5A在全部31例患儿的有神经节细胞的肠组织及无神经节细胞的肠组织中均有表达,但在无神经节细胞的肠组织中,Semaphorin5A的表达水平增高(P0.05)。结论：Semaphorin5A的表达在先天性巨结肠症患儿的无神经节细胞的肠组织中表达增高,提示Semaphorin5A可能为先天性巨结肠症的危险因素之一。
[Abstract]:Objective: Hirschsprung disease (HSCR) is a disease characterized by the absence of ganglion cells in the intermuscular plexus of the intestine. At present, it is known that the abnormal migration of intestinal neuron precursor cells (ENPs) is one of the pathogenesis of Hirschsprung's disease, and Semaphorin5A plays an important role in the migration of ENPs. This study attempts to analyze the expression of Semaphorin5A protein in children with Hirschsprung's disease and to explore the relationship between Semaphorin5A and Hirschsprung's disease. Methods: a total of 31 children with Hirschsprung's disease underwent surgical treatment in Shenzhen Children's Hospital. The intestinal tissues of children with Hirschsprung's disease were divided into experimental group and control group. The specimens of the experimental group were confirmed by pathological examination. The specimens of the control group were confirmed to contain ganglion by pathological examination. Immunofluorescence and Western-blot techniques were used to detect the expression of Semaphorin5A in ganglion cells and non-ganglion cells in all children, and the difference was statistically significant by t-test analysis (P03.05). Results: the results of immunofluorescence showed that Semaphorin5A could be expressed in ganglion cells and submucosal cells in the myenteric layer of all specimens. Western-blot analysis of ring and longitudinal muscles showed that Semaphorin5A was expressed in all 31 children with ganglion cells and without ganglion cells, but the expression level of Semaphorin 5A was increased in the intestinal tissues without ganglion cells. Conclusion the expression of Semaphorin5A in the intestine of children with Hirschsprung's disease is increased, suggesting that Semaphorin5A may be one of the risk factors of Hirschsprung's disease.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R726.5

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