钙卫蛋白和晚期糖基化终末产物受体在儿童过敏性紫癜中的表达研究

发布时间：2018-06-07 13:17

本文选题：钙卫蛋白 + 晚期糖基化终末产物受体　；参考：《南京医科大学学报(自然科学版)》2017年03期

【摘要】：目的 :研究过敏性紫癜(Henoch-Schoenlein purpura,HSP)患儿外周血钙卫蛋白(calprotectin,CP)、晚期糖基化终末产物受体(receptor of advanced glycosylation end products,RAGE)、超敏C反应蛋白(high sensitivity C-reactive protein,hs-CRP)的表达情况,探讨CP在HSP患儿中的意义。方法:选取HSP儿童(HSP组)36例和正常健康儿童(对照组)17例。采用酶联免疫吸附法(ELISA)检测血清CP的水平,应用逆转录-聚合酶链反应检测外周血单个核细胞中RAGE m RNA表达水平,全自动生化分析仪检测血清hs-CRP水平。结果:HSP患儿血清CP表达量(22.51±17.28)μg/L,低于对照儿童(67.73±15.15)μg/L,差异有统计学意义(P0.001);HSP组和对照组儿童RAGE m RNA表达量差异无统计学意义(P0.05);HSP患儿血清hs-CRP表达量(11.16±6.70)mg/L,高于对照儿童(1.59±1.02)mg/L,差异有统计学意义(P0.001)。混合型HSP患儿CP表达量(19.14±14.11)μg/L低于单纯型患儿(43.90±23.44)μg/L,差异有统计学意义(P0.05);混合型HSP患儿RAGE m RNA表达量与单纯型相比差异无统计学意义(P0.05);混合型HSP患儿hs-CRP表达量(12.80±6.59)mg/L较单纯型(5.95±2.65)mg/L增高,差异有统计学意义(P0.05)。HSP患儿的CP、RAGE、hs-CRP的表达量均无性别差异。HSP患儿CP、RAGE及hs-CRP的表达量均无明显相关性。结论:HSP患儿CP低表达。在HSP中,RAGE不是CP发挥作用的主要受体。
[Abstract]:Objective: to study the expression of calprotectinophane, advanced glycation end product receptor of advanced glycosylation end products ragger, and high sensitivity C-reactive protein hs-CRPs in children with Henoch-Schoenlein purpura HSPs, and to explore the significance of CP in children with HSP. Methods: 36 children with HSP and 17 healthy children (control group) were selected. Serum CP level was detected by Elisa, RAGE m RNA expression in peripheral blood mononuclear cells was detected by reverse transcription-polymerase chain reaction (RT-PCR), serum hs-CRP level was detected by automatic biochemical analyzer. Results the serum CP expression in children with RAGE m RNA was 22.51 卤17.28 渭 g / L, which was lower than that in control children (67.73 卤15.15) 渭 g / L. There was no significant difference in the expression of RAGE m RNA between the HSPgroup and the control group (P 0.05). The expression of hs-CRP in serum was 11.16 卤6.70 mg / L in the control group and 1.59 卤1.02mg / L in the control group, and the difference was statistically significant (P 0.001). The expression of CP in mixed HSP was 19.14 卤14.11 渭 g / L lower than that in simple type (43.90 卤23.44) 渭 g / L, the difference was statistically significant (P 0.05), there was no significant difference in RAGE m RNA expression between mixed type and simple type (P 0.05), and the expression of hs-CRP in mixed type of HSP was 12.80 卤6.59)mg/L higher than that in simple type of HSP 5.95 卤2.65)mg/L. There was no significant difference in the expression of hs-CRP and CPRAGEHs-CRP in children with HSP. there was no significant correlation between the expression of CPG RAGEHs-CRP and the expression of CPRAGE and hs-CRP in HSP2 children. Conclusion CP expression is low in children with HSP. Rage is not the main receptor of CP in HSP.
【作者单位】：扬州大学附属泰兴医院儿科;
【分类号】：R725.5

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