早期过度喂养和高脂膳食对大鼠能量代谢的影响

发布时间：2018-06-08 00:08

本文选题：早期营养 + 肥胖　；参考：《营养学报》2017年03期

【摘要】：目的探讨早期不同营养方式喂养对大鼠能量代谢和行为的影响。方法应用小窝鼠模型模拟哺乳期营养过度的环境,形成过度喂养组(small litter,SL组,每窝3只)或正常喂养组(normal litter,NL组,每窝10只)。3w断奶后给予高脂膳食喂养至13w模拟断乳后过度喂养环境,分为正常喂养组(NL)、哺乳期过度喂养组(SL)、断乳后过度喂养组(NL-HF)和持续过度喂养组(SL-HF)共4组。每周测量体质量及摄食量,分别于3w及13w用间接测热法检测大鼠总能量消耗,代谢测量分析系统分析大鼠摄食行为及运动情况。结果断乳时SL组总能量消耗显著低于NL组(P0.05),且持续至成年,而两组运动量无明显差异(P0.05)。断乳后高脂膳食也显著降低大鼠能量消耗水平,同时大鼠总运动量显著降低(P0.05),而持续过度喂养组大鼠能量摄入、单餐摄食量及进食速度均明显高于其他各组大鼠(P0.05)。结论哺乳期过度喂养和断乳后高脂饮食均降低机体的能量代谢水平。而持续过度喂养大鼠还出现明显的行为改变,导致更为严重的肥胖和胰岛素抵抗。
[Abstract]:Objective to investigate the effects of early nutrition on energy metabolism and behavior in rats. Methods the litter rat model was used to simulate the environment of lactation over nutrition, and to form a small litter SL group (3 rats per litter) or a normal litternacle NL group (n = 3) or a normal feeding group (n = 3) or a normal litterliter NL group (n = 3). Ten rats in each litter were fed with high fat diet after weaning for 13 weeks. They were divided into 4 groups: normal feeding group (NLL), lactation overfeeding group (SLL), postweaning overfeeding group (NL-HFR) and continuous overfeeding group (SL-HFR). Body mass and food intake were measured weekly. The total energy consumption was measured by indirect calorimetry at 3w and 13w respectively. The feeding behavior and movement of rats were analyzed by metabolic measurement and analysis system. Results during weaning, the total energy expenditure in SL group was significantly lower than that in NL group (P 0.05) and lasted until adulthood, but there was no significant difference in exercise volume between the two groups. After weaning, the high fat diet also significantly reduced the energy consumption of rats, and the total exercise amount of rats decreased significantly (P 0.05), while the energy intake, single meal intake and feeding rate of the rats in the group of continuous overfeeding were significantly higher than those in the other groups. Conclusion during lactation and high fat diet after weaning, energy metabolism was decreased. There were also significant behavioral changes in sustained overfeeding, leading to more severe obesity and insulin resistance.
【作者单位】：南京医科大学附属儿童医院儿童保健科;
【基金】：国家自然科学基金(No.81273064)
【分类号】：R723.14

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