瘦素对早产儿脑白质损伤模型大鼠远期空间记忆能力的影响

发布时间：2018-06-08 17:21

本文选题：瘦素 + 脑白质损伤　；参考：《中国当代儿科杂志》2017年12期

【摘要】：目的通过观察瘦素对早产儿脑白质损伤模型大鼠空间记忆的影响,探索其神经保护作用。方法将80只新生大鼠随机分为假手术组(n=27)、模型组(n=27)和瘦素干预组(n=26),模型组和瘦素干预组大鼠制备早产儿脑白质损伤模型,瘦素干预组于造模后立即给予生理盐水稀释的瘦素(100 μg/kg),连续4 d。观察各组大鼠存活情况并监测体重变化,21日龄后通过Morris水迷宫实验观察各组大鼠空间记忆能力。结果各组新生大鼠存活率比较差异无统计学意义(P0.05)。至生后10 d内,瘦素干预组大鼠体重接近假手术组,但低于模型组(P0.05);10 d后瘦素干预组生长速度加快,体重超过模型组和假手术组,但差异无统计学意义(P0.05)。定位航行训练结果显示:实验第2天开始,各组平台潜伏期比较差异有统计学意义(P0.05);从第4天开始,瘦素干预组平台潜伏期接近假手术组,短于模型组(P0.05)。空间搜索实验结果显示:与假手术组比较,模型组穿越平台次数减少,平台潜伏期延长(P0.05);与模型组比较,瘦素干预组穿越平台次数增加,平台潜伏期缩短(P0.05),且与假手术组比较差异均无统计学意义(P0.05)。结论瘦素干预可以减轻早产儿脑白质损伤的空间记忆障碍,对早产儿脑损伤具有神经保护作用,值得进一步研究。
[Abstract]:Objective To observe the effect of leptin on spatial memory of rat brain white matter injury model in premature infants and explore its neuroprotective effect. Methods 80 newborn rats were randomly divided into sham operation group (n=27), model group (n=27) and leptin intervention group (n=26), model group and leptin intervention group to prepare brain white matter damage model in preterm infants and leptin intervention group. After making the model, the thinning element (100 g/kg) diluted with saline was given, and the survival of the rats in each group was observed and the weight changes were monitored by 4 d.. After 21 days of age, the spatial memory ability of the rats was observed by the Morris water maze test. The survival rate of the newborn rats was not statistically significant (P0.05). The intervention of leptin intervention was within 10 d after birth. The weight of the rats in the group was close to the sham group, but it was lower than the model group (P0.05). The growth speed of the leptin intervention group was faster after 10 d, and the weight exceeded the model group and the sham operation group, but the difference was not statistically significant (P0.05). The results of the navigation training showed that the incubation period of the flat table was statistically significant (P0.05) from the beginning of the experiment (P0.05); from the fourth day. The latency of the leptin intervention group was close to the sham operation group and shorter than the model group (P0.05). The results of space search showed that compared with the sham group, the number of crossing platform in the model group was reduced and the latency of the platform was prolonged (P0.05). Compared with the model group, the number of leptin intervention groups increased and the latency of the platform shortened (P0.05), and compared with the sham operation group. There is no significant difference between the difference and the difference (P0.05). Conclusion leptin intervention can reduce the spatial memory impairment of premature brain white matter injury and have neuroprotective effect on premature infants' brain injury, which deserves further study.
【作者单位】：东南大学生物科学与医学工程学院;东南大学附属中大医院儿科;
【基金】：国家自然科学基金(81771628)
【分类号】：R722.6

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