卵清蛋白诱导的过敏小鼠模型中多不饱和脂肪酸代谢变化

发布时间：2018-06-30 08:29

本文选题：n-多不饱和脂肪酸 + 卵清蛋白　；参考：《中国循证儿科杂志》2017年03期

【摘要】：目的观察卵清蛋白(OVA)诱导的过敏小鼠模型中多不饱和脂肪酸(PUFA)代谢的变化。方法以缺乏n-3 PUFA饲料饲养雌鼠的仔鼠(21日龄)24只,随机分为空白组、OVA组和PBS组,各8只。实验第1 d,空白组心脏采血处死。实验第1和15 d,OVA组腹腔注射含50μg OVA和1.3 mg氢氧化铝凝胶的PBS 0.2 mL,PBS组腹腔注射等量PBS。实验第29~39 d,OVA组以含50 mg OVA的PBS 0.3 mL隔日灌胃,共6次;PBS组予等量PBS灌胃。实验第39 d末次灌胃后,OVA组和PBS组均心脏采血处死,取空肠组织和脾组织。OVA过敏小鼠模型成功建立的判断标准:观察灌胃后小鼠腹泻情况,对空肠组织切片进行形态学观察(HE染色)和肥大细胞计数(甲苯胺蓝染色),血清sIgE水平检测(ELISA法),脾组织中IL-4和IFN-γ检测(ELISA法)。提取各组小鼠血清中的脂肪酸,采用液相色谱法检测α-亚麻酸(ALA)、二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、花生四烯酸(AA)和亚油酸(LA)水平。结果 (1)成功建立OVA诱导过敏小鼠模型:与PBS组比较,OVA组在灌胃后发生急性腹泻;小肠绒毛有大量炎症细胞浸润,潘氏细胞脱颗粒,固有层中肥大细胞聚集脱颗粒,肥大细胞数目增加(P0.05);血清OVA-sI gE水平、脾细胞培养上清中IL-4和IL-4/IFN-γ升高(P0.05)。(2)PBS组血清DHA、EPA和LA水平较空白组降低,AA水平升高,差异均有统计学意义,OVA组较PBS组血清DHA水平降低。结论 OVA过敏小鼠出现以DHA降低为主的脂肪酸代谢紊乱。
[Abstract]:Objective to observe the metabolic changes of polyunsaturated fatty acids (PUFA) in allergic mice induced by ovalbumin (OVA). Methods Twenty-four female rats (21 days old) fed with n-3 PUFA were randomly divided into control group (OVA group) and PBS group (n = 8). On the first day of the experiment, the heart of the blank group was collected and killed. In the first and fifteenth days, the same amount of PBSs was injected intraperitoneally into the PBS 0.2 mL PBS group containing 50 渭 g OVA and 1.3 mg aluminum hydroxide gel. In the 29 ~ (th) ~ (th) day, the OVA group was perfused with 0.3 mL PBS containing 50 mg OVA every other day, and the same amount of PBS was given to the PBS group 6 times a day. At the end of the 39th day of the experiment, the OVA group and PBS group were collected and executed in the heart. The criteria for the successful establishment of the mice model of jejunum and splenic tissue were as follows: the diarrhea of mice after gastric perfusion was observed. Morphological observation (HE staining), mast cell count (toluidine blue staining), serum Siga E level (Elisa), IL-4 and IFN- 纬 in spleen tissue were observed. The levels of 伪 -linolenic acid (Ala), eicosapentaenoic acid (EPA), 22 carbohexaenoic acid (DHA), arachidonic acid (AA) and linoleic acid (LA) were determined by liquid chromatography. Results (1) the model of allergic mice induced by OVA was successfully established: compared with PBS group, acute diarrhea occurred in the OVA group, there were a large number of inflammatory cells infiltrating in the villi of the small intestine, Panzhix cells degranulated, and mast cells gathered and degranulated in the lamina propria. The number of mast cells increased (P0.05), the levels of serum OVA-sIgE, IL-4 and IL-4 / IFN- 纬 in the supernatant of splenocyte culture were increased (P0.05). (2), the levels of DHAA EPA and LA in PBS group were lower than those in control group (P0.05), the difference was statistically significant (P < 0.05) compared with PBS group, the level of serum DHA in OVA group was lower than that in PBS group. Conclusion OVA hypersensitive mice have fatty acid metabolic disorder with DHA decreasing.
【作者单位】：重庆医科大学附属儿童医院儿保科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室;
【分类号】：R-332;R725.9

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