EV71感染患儿TLR7基因多态性及抗病毒免疫研究

发布时间：2018-07-03 11:43

本文选题：Toll样受体7 + 肠道病毒71型　；参考：《遵义医学院》2014年硕士论文

【摘要】：目的：探讨肠道病毒71型(Enterovirus71，EV71)感染重症患儿Toll样受体7(Toll-likereceptor7，TLR7)及其基因多态性与抗病毒免疫、病情和预后的关系。方法：研究对象包括深圳市儿童医院收治的2012年至2013年EV71感染患儿115例（按病情分为重症组和危重症组），以及同期同年龄正常组婴幼儿120例。提取研究对象外周血基因组DNA，PCR扩增TLR7基因外显子及启动子区序列，扩增产物直接测序，分析测序结果；流式细胞术检测外周血单核细胞（monocyte，MC）TLR7平均荧光强度表达；流式细胞术微球阵列法（cytometric bead array，CBA）检测血浆IFN-α、IL-6、IL-10、IL-12等细胞因子的浓度。结果：（1）18例EV71感染患儿中TLR7基因3号外显子发现c.371TA位点突变，其基因型为半合子。（2）EV71感染危重症患儿外周血MC TLR7平均荧光强度表达明显高于同期同龄正常婴幼儿（p0.01），，比较重症患儿间TLR7平均荧光强度变化无明显区别（p0.05）。（3）EV71感染患儿细胞因子IL-12及IFN-a水平明显高于同期正常组婴幼儿（p0.01），IL-6和IL-10细胞因子浓度无明显改变（p0.05）。结论：TLR7基因多态性可能参与了EV71感染的发病过程，TLR7基因3号外显子c.371TA位点突变可能为EV71的易感基因。TLR7介导了EV71感染所致免疫功能的紊乱，引起EV71感染患儿IL-12及IFN-a细胞因子水平增高。
[Abstract]:Objective: to investigate the association of Toll-like receptor 7 (TLR7) gene polymorphism with antiviral immunity, condition and prognosis in severe children infected with enterovirus 71 (Enterovirus71). Methods: 115 children with EV71 infection were treated in Shenzhen Children's Hospital from 2012 to 2013 (divided into critical and critical groups according to their condition) and 120 infants of the same age in the same age group. The exons and promoters of TLR7 gene were amplified by PCR from peripheral blood genomic DNA, the products were sequenced directly and the results of sequencing were analyzed, and the mean fluorescence intensity of TLR7 in peripheral blood monocytes (monocyte MC) was detected by flow cytometry. Plasma levels of cytokines such as IL-6, IL-10 and IL-12 were measured by flow cytometry with (cytometric bead array. Results: (1) the mutation of c. 371TA in exon 3 of TLR7 gene was found in 18 children with EV71 infection. (2) the mean fluorescence intensity of peripheral blood MC TLR7 in severe children with EV71 infection was significantly higher than that in normal infants of the same age (p0.01). There was no significant difference in the mean fluorescence intensity of TLR7 between the severe children (p0.05). (3) and the children with EV71 infection (p0.05). (3). The levels of cytokines IL-12 and IFN-a were significantly higher than those of normal infants (p0.01) and the concentrations of IL-6 and IL-10 had no significant change (p0.05). Conclusion the gene polymorphism of TLR7 may be involved in the pathogenesis of EV71 infection. The mutation of c 371TA in exon 3 of TLR7 gene may be the susceptible gene of EV71. TLR7 mediates the disorder of immune function caused by EV71 infection. The levels of IL-12 and IFN-a cytokines were increased in children with EV 71 infection.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R725.1

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