支气管肺泡灌洗液G试验可有效诊断早产儿肺部侵袭性真菌感染

发布时间：2018-07-11 10:04

本文选题：支气管肺泡灌洗液 + 早产儿　；参考：《基因组学与应用生物学》2017年07期

【摘要】：本研究试图通过测量血清与支气管肺泡灌洗液中1,3-β-D葡聚糖浓度,分析应用支气管肺泡灌洗液G试验作为早期诊断早产儿肺部侵袭性真菌感染的可行性。选取2014年2月至2016年9月经确诊为侵袭性真菌感染的早产患儿42例作为观察组,将同期生产于我院的健康早产儿61例作为对照组。在清晨时间采集观察组与对照组早产儿的静脉血与分支气管肺泡灌洗液,将静脉血离心取上层血清待用,比较血清与支气管肺泡灌洗液的G试验的表达情况、阳性率及灵敏性。观察组早产儿经测量后,支气管肺泡灌洗液以及血浆中的1,3-β-D葡聚糖水平与健康早产儿比较有差异,说明通过G实验检测1,3-β-D葡聚糖有诊断早产儿侵袭性真菌感染的价值;观察组早产儿的支气管肺泡灌洗液G试验阳性率为83.33%,血浆G试阳性率为64.29%,x~2值为3.941,p值为0.047;支气管肺泡灌洗液G试验灵敏度为89.47%,血浆G试验灵敏度为65.79%,二者x~2值为6.138,p值为0.013。本研究的结果认为支气管肺泡灌洗液G试验可以有效、敏感的测定早产儿体内1,3-β-D葡聚糖的表达情况,应用于早产儿早期诊断肺部侵袭性真菌感染具有临床价值。
[Abstract]:The aim of this study was to analyze the feasibility of using bronchoalveolar lavage fluid G test for early diagnosis of invasive fungal infection in the lungs of premature infants by measuring the concentration of 1t3- 尾 -D glucan in serum and bronchoalveolar lavage fluid (BALF). From February 2014 to September 2016, 42 preterm infants with invasive fungal infection were selected as observation group, and 61 healthy premature infants born in our hospital during the same period as control group. The venous blood and bronchoalveolar lavage fluid (BALF) of premature infants in the observation group and the control group were collected in the early morning. The upper serum was centrifuged for use. The expression, positive rate and sensitivity of G-test between serum and bronchoalveolar lavage fluid were compared. Compared with the healthy preterm infants, the levels of 1t3- 尾 -D glucan in bronchoalveolar lavage fluid and plasma in the observation group were different from those in the healthy premature infants, indicating that the detection of 1t3- 尾 -D-glucan by G test was valuable in the diagnosis of invasive fungal infection in preterm infants. The positive rate of G-test in bronchoalveolar lavage fluid (BALF) was 83.33 in the observation group, the positive rate of plasma G-test was 64.29 and 3.941 (p = 0.047), the sensitivity of bronchoalveolar lavage fluid (BALF) was 89.47, the sensitivity of plasma G-test was 65.79 and the value of plasma G-test was 6.138p (0.0138p). The results of this study suggest that the bronchoalveolar lavage fluid G test can be used to detect the expression of 1k3- 尾 -D glucan in premature infants, and it has clinical value in the early diagnosis of pulmonary invasive fungal infection in premature infants.
【作者单位】：佛山市妇幼保健院;
【基金】：佛山市妇幼保健院的资助
【分类号】：R725.1

【参考文献】