中性粒细胞CD64和降钙素原联合检测在新生儿细菌感染早期诊断中的价值

发布时间：2018-07-12 11:49

本文选题：CD + 降钙素原　；参考：《中国当代儿科杂志》2017年08期

【摘要】：目的探讨血清中性粒细胞CD64和降钙素原(PCT)联合检测在新生儿细菌感染早期诊断中的价值。方法将37例细菌感染新生儿依据出院诊断分为败血症组(n=15)和一般感染组(非败血症患儿;n=22);并选取同期住院非感染新生儿作为对照组(n=21)。各组新生儿均于入院后即刻抽取静脉血,采用流式细胞术检测血清中性粒细胞CD64表达,化学发光法和免疫透射比浊法分别检测血清PCT及CRP水平。结果败血症组血清中性粒细胞CD64、PCT、CRP水平高于对照组(P0.01);一般感染组中性粒细胞CD64水平高于对照组(P0.01);败血症组血清PCT、CRP水平高于一般感染组(P0.01)。中性粒细胞CD64、PCT、CRP诊断细菌感染的曲线下面积分别为0.818、0.818、0.704,均低于中性粒细胞CD64与PCT联合诊断细菌感染的曲线下面积(0.926)。中性粒细胞CD64与PCT联合检测在早期诊断新生儿感染的灵敏度和准确度分别为97.29%和89.65%,较CRP联合中性粒细胞CD64或PCT检测的灵敏度和准确度均高,较中性粒细胞CD64、PCT及CRP单项检测的灵敏度和准确度更高。结论中性粒细胞CD64、PCT联合检测能显著提高新生儿细菌感染诊断的灵敏度及准确度,有助于早期识别细菌感染。
[Abstract]:Objective to investigate the value of serum neutrophil CD64 and procalcitonin (PCT) in the early diagnosis of neonatal bacterial infection. Methods 37 newborns with bacterial infection were divided into septicemia group (n = 15) and general infection group (n = 22) according to the diagnosis of discharge, and non-infective newborns in the same period were selected as control group (n = 21). The expression of CD64 in neutrophils was detected by flow cytometry and the levels of serum PCT and CRP were detected by chemiluminescence and immunoturbidimetry respectively. Results the levels of serum neutrophils, neutrophil CD64 and PCTnc-CRP in septicemia group were higher than those in control group (P0.01), and those in general infection group were higher than those in control group (P0.01). The areas under the curve for the diagnosis of bacterial infection were 0.818 ~ 0.818 ~ 0.704, respectively, which were lower than those for the co-diagnosis of bacterial infection by neutrophil CD64 and PCT (0.926). The sensitivity and accuracy of combined detection of neutrophil CD64 and PCT in early diagnosis of neonatal infection were 97.29% and 89.65 respectively, which were higher than that of CRP combined with neutrophil CD64 or PCT. The sensitivity and accuracy of PCT and CRP were higher than that of neutrophil CD64 and CRP. Conclusion the combined detection of neutrophil CD64 and PCT can significantly improve the sensitivity and accuracy of neonatal bacterial infection diagnosis, and is helpful for early identification of bacterial infection.
【作者单位】：皖南医学院第一附属医院儿科;皖南医学院第一附属医院中心实验室;
【分类号】：R722.13

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