IL-2及IL-2Rα基因多态性与汉族儿童支气管哮喘的关联
发布时间：2018-07-17 02:25
【摘要】：目的探讨IL-2(rs6822844)及IL-2Rα(rs2104286)基因多态性和汉族儿童支气管哮喘发生的关联。方法随机选取144例支气管哮喘患儿为病例组,228例健康儿童为对照组。用实时荧光定量聚合酶链反应(q RT-PCR)直接测序鉴定各位点基因型;同时采用q RT-PCR检测对照组IL-2Rα各基因型外周血IL-2Rα信使核糖核酸(m RNA)相对表达量,采用酶联免疫吸附试验(ELISA)检测各基因型血浆s IL-2Rα水平。结果IL-2Rα(rs2104286)位点等位基因A在病例组分布频率(86.8%)高于对照组(79.4%)(OR=1.708,95%CI:1.113,2.629,P=0.010),病例组中AA基因型分布频率(75.0%)高于对照组(62.3%)(OR=1.745,95%CI:1.058,2.884,P=0.021);未发现IL-2(rs6822844)位点有多态性分布。对照组中IL-2Rα(rs2104286)位点AA基因型个体外周血IL-2Rαm RNA相对表达量以及血浆s IL-2Rα水平均高于AG基因型(Z=-2.029和-2.361,P=0.043和0.018)。结论 IL-2Rα(rs2104286)基因多态性可能与汉族儿童支气管哮喘发生相关,而且该位点基因型可影响其转录及表达。
[Abstract]:Objective to investigate the association of interleukin-2 (rs6822844) and IL-2R 伪 (rs2104286) gene polymorphisms with bronchial asthma in children of Han nationality. Methods 144 children with bronchial asthma were randomly selected as case group and 228 healthy children as control group. The locus genotypes were identified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the relative expression of IL-2R 伪 messenger ribonucleic acid (mRNA) in peripheral blood of the control group was detected by qRT-PCR. Enzyme linked immunosorbent assay (Elisa) was used to detect the levels of plasma sIL-2R 伪 in all genotypes. Results the distribution frequency of IL-2R 伪 (rs2104286) allele A in the case group (86.8%) was higher than that in the control group (79.4%) (OR1. 708%). The distribution frequency of AA genotype in the case group (75.0%) was higher than that in the control group (62.3%). There was no polymorphic distribution of IL-2 (rs6822844) locus. The relative expression of IL-2R 伪 mRNA in peripheral blood and the level of plasma sIL-2R 伪 in AA genotype at IL-2R 伪 (rs2104286) locus were significantly higher than those in AG genotype (Z ~ (-2.029) and -2.361) (0.043 and 0.018). Conclusion the polymorphism of IL-2R 伪 (rs2104286) gene may be associated with bronchial asthma in Han children, and the genotype of IL-2R 伪 (rs2104286) gene may affect its transcription and expression.
【作者单位】： 浙江省金华市中医医院检验科;浙江省杭州市第一人民医院检验科;
【分类号】：R725.6
本文编号：2128601
[Abstract]:Objective to investigate the association of interleukin-2 (rs6822844) and IL-2R 伪 (rs2104286) gene polymorphisms with bronchial asthma in children of Han nationality. Methods 144 children with bronchial asthma were randomly selected as case group and 228 healthy children as control group. The locus genotypes were identified by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), and the relative expression of IL-2R 伪 messenger ribonucleic acid (mRNA) in peripheral blood of the control group was detected by qRT-PCR. Enzyme linked immunosorbent assay (Elisa) was used to detect the levels of plasma sIL-2R 伪 in all genotypes. Results the distribution frequency of IL-2R 伪 (rs2104286) allele A in the case group (86.8%) was higher than that in the control group (79.4%) (OR1. 708%). The distribution frequency of AA genotype in the case group (75.0%) was higher than that in the control group (62.3%). There was no polymorphic distribution of IL-2 (rs6822844) locus. The relative expression of IL-2R 伪 mRNA in peripheral blood and the level of plasma sIL-2R 伪 in AA genotype at IL-2R 伪 (rs2104286) locus were significantly higher than those in AG genotype (Z ~ (-2.029) and -2.361) (0.043 and 0.018). Conclusion the polymorphism of IL-2R 伪 (rs2104286) gene may be associated with bronchial asthma in Han children, and the genotype of IL-2R 伪 (rs2104286) gene may affect its transcription and expression.
【作者单位】： 浙江省金华市中医医院检验科;浙江省杭州市第一人民医院检验科;
【分类号】：R725.6
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