呼吸道合胞病毒感染后期OVA刺激对小鼠气道炎症及AHR的影响

发布时间：2018-07-24 11:59

【摘要】：目的研究呼吸道合胞病毒(RSV)感染后期过敏原刺激对小鼠气道炎症及AHR的影响。方法 6~8周龄雌性Balb/c小鼠随机分为Mock组、RSV组、OVA组及R+O组。检测各组小鼠支气管肺泡灌洗液(BALF)中细胞总数及分类计数;苏木精-伊红(HE)染色观察肺部病理损伤;全身体积描述记法检测小鼠气道反应性;ELISA检测BALF中IL-4、IL-5、CXCL10、CCL5、CCL20的水平。结果 R+O组小鼠BALF中炎症细胞总数、巨噬细胞、中性粒细胞及嗜酸性粒细胞分类计数较其他3组均显著增高(P0.05);R+O组小鼠肺组织病理评分及AHR较其他3组亦显著增高(P0.05);BALF中各处理组IL-4、IL-5较Mock组明显增高(P0.05),但处理组之间无显著性差异;R+O组CXCL10水平较其他3组显著升高(P0.05)。结论 RSV感染后期接受OVA刺激可致CXCL10表达增加,进而促使巨噬细胞、中性粒细胞及嗜酸性粒细胞募集,加重小鼠气道炎症及AHR。
[Abstract]:Objective to study the effects of allergen stimulation of respiratory syncytial virus (RSV) infection on airway inflammation and AHR in mice. Methods female Balb/c mice aged 6 to 8 weeks were randomly divided into Mock group and RO group. The total number of cells in the bronchoalveolar lavage fluid (BALF) and their classification count, hematoxylin-eosin (HE) staining, and the level of IL-4, IL-5, CXCL10 and CCL5CCL20 in BALF were detected by whole body volume description method. Results the total number of inflammatory cells and macrophages in BALF of rats in group R O, The number of neutrophil and eosinophil were significantly higher than that of the other three groups (P0.05). The pathological score of lung tissue and the AHR of the mice in the control group were also significantly higher than those in the other three groups (P0.05). The IL-4 IL-5 of the treated group was significantly higher than that of the Mock group (P0.05), but the treatment group was significantly higher than that of the other three groups (P0.05). The level of CXCL10 in R O group was significantly higher than that in the other three groups (P0.05). Conclusion in the later stage of RSV infection, OVA stimulation can increase the expression of CXCL10, and promote the recruitment of macrophages, neutrophils and eosinophils, and aggravate the airway inflammation and AHR in mice.
【作者单位】：重庆医科大学附属儿童医院儿科研究所儿科学重庆市重点实验室/儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地;重庆医科大学附属儿童医院呼吸中心;
【基金】：国家自然科学基金(81170010,81470208)
【分类号】：R725.6

【相似文献】