人博卡病毒载量与儿童急性下呼吸道感染临床特征相关性研究

发布时间：2018-07-25 12:10

【摘要】：目的了解人类博卡病毒(HBoV)在儿童急性下呼吸道感染中的检出情况,并探讨HBoV载量与儿童急性下呼吸道感染临床特征的相关性。方法收集2011年3月至2014年3月因急性下呼吸道感染住院患儿的鼻咽抽吸物(NPAs)1 554份,采用实时荧光定量PCR检测12种RNA病毒和ADV、HBoV 2种DNA病毒,并同时检测HBoV阳性患儿的病毒载量。结果 1 554份标本中1 212份(77.99%)检出病毒,275份(17.70%)HBoV检出阳性。HBoV阳性病例中3岁者占94.9%,男性多于女性。275例HBoV阳性病例中,单一感染45例(16.36%),混合感染230例(83.64%),两者病毒载量的差异无统计学意义(P0.05)。有发热症状的HBoV阳性患儿病毒载量高于无发热者,有喘息症状的病毒载量高于无喘息者,差异均有统计学意义(P0.05)。轻、中、重度组急性下呼吸道感染患儿之间的病毒载量差异无统计学意义(P0.05)。结论 HBoV是儿童急性下呼吸道感染重要病原之一。病毒载量高的患儿,临床更易出现发热、喘息等症状,但疾病严重程度及混合感染与病毒载量之间无显著相关性。
[Abstract]:Objective to investigate the detection of human Boca virus (HBoV) in children with acute lower respiratory tract infection and to explore the correlation between HBoV load and clinical features of acute lower respiratory tract infection in children. Methods from March 2011 to March 2014, a total of 1,554 nasopharyngeal aspirates (NPAs) were collected from hospitalized children with acute lower respiratory tract infection. Twelve RNA viruses and two DNA viruses were detected by real-time fluorescence quantitative PCR. The viral load of HBoV positive children was also detected. Results among the 1 554 specimens, 1 212 (77.99%) were positive for HBoV and 94.9% (17.70%) were positive for HBoV, and 94.9% were found in male than in female, respectively. There were 45 cases (16.36%) with single infection and 230 cases (83.64%) with mixed infection. There was no significant difference in viral load between the two groups (P0.05). The viral load of HBoV positive children with febrile symptoms was higher than that of non-febrile children, and the viral load with wheezing symptoms was higher than that without wheezing (P0.05). There was no significant difference in viral load between mild, moderate and severe children with acute lower respiratory tract infection (P0.05). Conclusion HBoV is one of the important pathogens of acute lower respiratory tract infection in children. Children with high viral load were more likely to have symptoms such as fever and wheezing, but there was no significant correlation between the severity of disease and mixed infection and viral load.
【作者单位】：湖南省人民医院儿童医学中心;
【基金】：国家科技重大专项项目(2014ZX10004002-007-004)
【分类号】：R725.6

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