过敏性紫癜患儿急性期血浆sCD40L水平变化及意义

发布时间：2018-08-05 09:53

【摘要】：目的探讨过敏性紫癜(HSP)患儿急性期血浆可溶性CD40配体(s CD40L)水平的变化及意义。方法选择急性期HSP患儿79例(HSP组),按有无肾脏损害进一步分为紫癜性肾炎组(HSPN)和非紫癜性肾炎组(NHSPN),另择体检健康儿童29例为对照组。采用免疫ELISA法测定血浆中s CD40L水平,采用免疫散射比浊法检测血浆免疫球蛋白(包括Ig G、Ig A、Ig M和Ig E)水平,采用流式细胞技术测定外周血中淋巴细胞亚群。Pearson相关检验进行相关性分析。结果与对照组比较,其余三组血浆s CD40L水平高(P均0.05);HSPN组高于NHSPN组(P0.05)。HSPN组外周血B细胞所占百分比高于NHSPN组(P均0.05)。相关分析结果显示,HSP患儿血浆s CD40L水平与外周血B细胞和血浆Ig A水平存在正相关性(r分别为0.516、0.322,P均0.05),与总T细胞、CD4+T细胞所占百分比、CD8+T细胞所占百分比、CD4+/CD8+、Ig M、Ig G、Ig E均无明显相关性(P均0.05)。结论HSP患儿急性期血浆s CD40L水平升高,s CD40L异常表达可能在HSP尤其是HSPN的免疫发病机制中发挥重要作用。
[Abstract]:Objective to investigate the changes and significance of plasma soluble CD40 ligand (s CD40L) in children with Henoch-Schonlein purpura (HSP). Methods Seventy-nine children with acute HSP (HSP group) were divided into two groups: Henoch-Schonlein nephritis group (HSPN) and non-Henoch-Schonlein purpura nephritis group (NHSPN),). 29 healthy children were selected as control group. The levels of s CD40L and immunoglobulin (Ig G G G Ag G M and Ig E) in plasma were measured by immuno ELISA assay and immunoturbidimetry respectively. Lymphocyte subsets in peripheral blood were determined by flow cytometry. Pearson correlation test was used to analyze the correlation. Results compared with the control group, the percentage of peripheral blood B cells in HSPN group was higher than that in NHSPN group (P0.05). The percentage of B cells in peripheral blood of HSPN group was higher than that of NHSPN group (P0. 05). Correlation analysis showed that there was a positive correlation between plasma s CD40L level and peripheral blood B cells and plasma IgA levels (r = 0. 516 卤0. 322, P < 0. 05, respectively), and a positive correlation with the percentage of total T cell CD 4 T cells and CD 8 T cells (P < 0. 05). There was no significant correlation between MG and IgE (P 0.05). Conclusion the abnormal expression of plasma s CD40L in children with HSP may play an important role in the immunopathogenesis of HSP, especially HSPN.
【作者单位】：青岛市妇女儿童医院;青岛大学附属医院;
【分类号】：R725.5

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