生命早期食物过敏对幼鼠内脏痛敏感性影响及结肠P物质异常表达在其形成中作用

发布时间：2018-08-26 13:12

【摘要】：目的建立SD幼鼠卵白蛋白(OVA)食物过敏(FA)模型；探讨FA对幼鼠内脏痛觉敏感性影响及结肠P物质(SP)异常表达在FA幼鼠内脏痛觉高敏形成中作用。方法3周龄雌性SD大鼠20只，随机分FA组和对照组(NS组)，，各10只。FA幼鼠通过低剂量OVA腹腔注射基础致敏及高剂量OVA灌胃激发建立SD幼鼠OVA-FA；检测血清OVA特异性IgE含量、观察肠黏膜病理改变及嗜酸性粒细胞、肥大细胞浸润情况进行幼鼠FA模型评价。通过观察两组幼鼠在不同压力结直肠扩张(CRD)刺激后的腹外斜肌(EOMA)放电测量进行内脏痛觉敏感性评价。应用免疫组织化学染色及计算机图像分析系统对结肠SP进行积分光密度值半定量分析。采用SPSS13.0软件包进行统计分析，α=0.05为显著性检验标准。结果FA组幼鼠出现不同程度竖毛，毛发失去光泽，活动减少或俯卧不动，抓耳挠腮或躁动不安及排不成形稀便；与对照组比较，FA组幼鼠血清OVA-IgE含量、空肠黏膜嗜酸性粒细胞和肥大细胞数及肥大细胞脱颗粒百分率均明显升高，差别有统计学意义(P均＜0.01)。在CRD为0、15、30、45、60、75mmHg时，FA组腹外斜肌放电幅值(uV)分别为17.74±0.72、18.63±1.72、22.55±1.70、28.63±7.00、33.97±7.34、37.26±8.4，NS组分别为17.43±1.18、17.27±1.16、17.73±1.42、19.55±3.54、23.29±5.46、25.20±4.75。随着CRD增加，腹外斜肌放电幅值较前明显升高，CRD30、45、60、75mmHg时，两组差别有统计学意义(P均＜0.01)。FA组、NS组幼鼠结肠SP积分光密度值分别为247.12±90.83、103.90±58.94，差别有统计学意义(P＜0.01)。结论通过低剂量OVA腹腔注射基础致敏及高剂量OVA灌胃激发可建立SD幼鼠FA模型。生命早期FA可导致幼鼠腹外斜肌放电幅值明显升高并伴有结肠SP表达增加，出现慢性内脏痛觉高敏感。生命早期FA导致的内脏痛觉高敏感性发生可能与肠黏膜肥大细胞表达增多、脱颗粒及肠道SP的异常表达有关。
[Abstract]:Objective to establish the (FA) model of food allergy to ovalbumin (OVA) in young SD rats and to investigate the effect of FA on visceral pain sensitivity and the abnormal expression of substance P (SP) in colon during the formation of visceral hyperalgesia Gao Min in FA young rats. Methods Twenty 3-week-old female SD rats were randomly divided into FA group (n = 10) and control group (NS group, n = 10). SD young rats were sensitized by low dose OVA intraperitoneal injection and stimulated by high dose OVA. OVA-FA; was used to detect OVA IgE content in serum of SD young rats. The pathological changes of intestinal mucosa and the infiltration of eosinophils and mast cells were observed to evaluate the FA model of young rats. The visceral pain sensitivity was evaluated by observing the (EOMA) discharges of the oblique ventral muscles of the two groups after (CRD) stimulation under different pressures. Immunohistochemical staining and computer image analysis system were used for semi-quantitative analysis of integrated optical density (OSD) of colon SP. SPSS13.0 software package was used for statistical analysis, 伪 -0. 05 was the significant test standard. Results the young rats in FA group had different degrees of hair erecting, hair losing luster, decreased activity or prone immobility, scratching of ear, restlessness or restlessness and defecation, compared with control group, the content of serum OVA-IgE in FA group was higher than that in control group. The number of eosinophilic granulocytes and mast cells and the percentage of degranulation of mast cells in jejunal mucosa were significantly increased (P < 0.01). When the CRD was 0 ~ 15 ~ 30 ~ 45 ~ 60 ~ 75mmHg, the (uV) of the external oblique muscle in the FA group was 17.74 卤0.72 ~ 18.63 卤1.72t 22.55 卤1.7028.63 卤7.34 卤37.97 卤7.34 ~ 37.26 卤8.4mm Hg, respectively, which was 17.43 卤1.1817.27 卤1.167.73 卤1.42n 19.55 卤3.523.29 卤5.46n 25.20 卤4.75 respectively. With the increase of CRD, the discharge amplitude of the oblique ventral muscle increased significantly (P < 0. 01). The SP optical density of colon in the FA group was 247.12 卤90.83103.90 卤58.94, respectively (P < 0. 01), and there was a significant difference between the two groups (P < 0. 01). Conclusion the FA model of young SD rats can be established by low dose intraperitoneal injection of OVA and high dose of OVA by intraperitoneal injection. Early FA could cause the amplitude of ventral obliquus muscle discharge and the expression of SP in colon increased, and the chronic visceral pain was hypersensitive. The high sensitivity of visceral pain induced by FA in early life may be related to the increased expression of mast cells in intestinal mucosa, degranulation and abnormal expression of intestinal SP.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R725.7;R-332

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