不同日龄新生大鼠缺氧缺血性脑损伤后脑组织谷氨酸含量变化的研究
发布时间：2018-08-29 14:03
【摘要】：目的：研究代表不同胎龄新生儿缺氧缺血性脑病(hypoxic ischemic encephalopathy, HIE)的模型鼠脑组织中谷氨酸(glutamate, Glu)含量变化的时间依从性及干预措施。方法：将128只新生SD大鼠按出生日龄(postnatal age, P)分为P2、P6、P12、P184组,每组32只；各组按不同干预措施分为缺氧缺血性脑损伤(hypoxic ischemic brain damage, HIBD)模型对照组(H)、α-氨基羟甲基恶唑丙酸(AMPA)干预组(A)、GYKI52466干预组(G)与空白对照组(N),每组8只。除N组外,各组参照Rice方法建立HIBD标准模型,A、G组于造摸后立即给药。用药6小时后采集标本,检测各组脑组织Glu含量,同时做病理学检查。结果：各日龄组脑组织中Glu含量比较,Glu含量与日龄呈负相关(r=-0.81,p0.01),P2组含量最高,与P6、P12、P18组比较均有显著性差异[(670.2±139.4)μmol/gprot.vs.(576±139) μmol/gprot.,(670.2±139.4)μmol/gprot.vs.(441.9±114.9) μmol/gprot.,(670.2±139.4)μmol/gprot.vs.(287.0±82.8) μmol/gprot.,Q=3.88,9.41,15.80,P0.05或P0.01]。各日龄组内比较,G组脑组织中Glu含量明显低于A组、H组和N组[(348.6±128.5)μmol/gprot. vs.(608.6±176.7)μmol/gprot.,(348.6±128.5)μmol/gprot.vs.(554.2±206.2)μmol/gprot.,(348.6±128.5)μmol/gprot.vs.(463.8±167.1) μmol/gprot.,Q=10.72,8.49,4.75,P均0.01]。病理学改变与G1u含量变化呈一致性。结论：代表不同胎龄新生儿脑发育水平的不同日龄新生大鼠脑组织中,Glu的含量具有时间依从性,与日龄呈负相关；HIBD可导致Glu含量明显增加而加重脑损伤；而GYKI52466可显著降低Glu含量,从而发挥神经保护作用,且胎龄越小,效果越明显。故应用GYKI52466早期干预可望有效防治因早产、窒息导致新生儿脑损伤。
[Abstract]:Aim: to study the temporal compliance and intervention of glutamate (glutamate, Glu) content in brain tissue of neonatal hypoxic-ischemic encephalopathy (hypoxic ischemic encephalopathy, HIE) model with different gestational ages. Methods: 128 neonatal SD rats were divided into two groups according to their birth age (postnatal age, P): P2P6 P12P184 group with 32 rats in each group. According to different intervention measures, each group was divided into (hypoxic ischemic brain damage, HIBD) model control group, (H), 伪 -aminomethyl oxazolyl propionate (AMPA) intervention group, (A) GYKI52466 intervention group (G) and control group (N), 8 rats in each group. With the exception of group N, the HIBD standard model was established by Rice in each group. After 6 hours of administration, the contents of Glu in brain tissue were measured, and pathological examination was made at the same time. 缁撴灉锛氬悇鏃ラ緞缁勮剳缁勭粐涓璆lu鍚噺姣旇緝,Glu鍚噺涓庢棩榫勫憟璐熺浉鍏，
本文编号：2211424
[Abstract]:Aim: to study the temporal compliance and intervention of glutamate (glutamate, Glu) content in brain tissue of neonatal hypoxic-ischemic encephalopathy (hypoxic ischemic encephalopathy, HIE) model with different gestational ages. Methods: 128 neonatal SD rats were divided into two groups according to their birth age (postnatal age, P): P2P6 P12P184 group with 32 rats in each group. According to different intervention measures, each group was divided into (hypoxic ischemic brain damage, HIBD) model control group, (H), 伪 -aminomethyl oxazolyl propionate (AMPA) intervention group, (A) GYKI52466 intervention group (G) and control group (N), 8 rats in each group. With the exception of group N, the HIBD standard model was established by Rice in each group. After 6 hours of administration, the contents of Glu in brain tissue were measured, and pathological examination was made at the same time. 缁撴灉锛氬悇鏃ラ緞缁勮剳缁勭粐涓璆lu鍚噺姣旇緝,Glu鍚噺涓庢棩榫勫憟璐熺浉鍏，
本文编号：2211424
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